Journal ArticleDOI
Reaction of manganese peroxidase of Bjerkandera adusta with synthetic lignin in acetone solution
TLDR
The reaction of manganese peroxidase (MnP) of the white-rot fungusBjerkandera adusta with synthetic lignin dehydrogenation polymer, DHP) in acetone medium was investigated and depolymerization of syringyl DHP was demonstrated.Abstract:
The reaction of manganese peroxidase (MnP) of the white-rot fungusBjerkandera adusta with synthetic lignin dehydrogenation polymer, DHP) in acetone medium was investigated. Gel-permeation chromatography of the DHP treated by MnP demonstrated depolymerization of syringyl DHP in the reaction mixture containing 70% acetone; moreover, concomitant repolymerization occurred to give highly polymerized products. Guaiacyl DHP was only repolymerized by MnP in the same acetone solution without giving degradation products. Addition of ascorbic acid to reaction mixtures containing acetone resulted in preferential depolymerization of syringyl DHP.read more
Citations
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Role of microbial enzymes in the bioremediation of pollutants: a review.
TL;DR: This review attempts to provide descriptive information on the enzymes from various microorganisms involved in the biodegradation of wide range of pollutants, applications, and suggestions required to overcome the limitations of their efficient use.
Journal Article
Research letterPolymerisation of lignins by ligninases from Phanerochaete chrysosporium
TL;DR: In this article, four major hemoproteins were purified by isoelectric focusing from an extracellular crude enzyme preparation, produced by the white rot fungus Phanerochaete chrysosporium under carbon-limited conditions.
Journal ArticleDOI
The synthesis and analysis of advanced lignin model polymers
TL;DR: In this article, the synthesis and characterisation of advanced lignin model polymers are described. Butler et al. showed that the controlled incorporation of the major linkages in Lignin is demonstrated to give complex hardwood and softwood LML polymers.
Journal ArticleDOI
Action of fungal laccases on lignin model compounds in organic solvents
Pekka Maijala,Maija-Liisa Mattinen,Paula Nousiainen,Jussi Kontro,Janne Asikkala,Jussi Sipilä,Liisa Viikari +6 more
TL;DR: The stability and reactivity of five different fungal laccases from the species Trametes hirsuta, Melanocarpus albomyces, Thielavia arenaria and Chaetomium thermophilum were investigated in the presence of organic solvents as mentioned in this paper.
Journal ArticleDOI
Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid
TL;DR: The results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lign in but also to its polymerization.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Lignin chemistry—past, present and future
TL;DR: Later development, beginning with the dehydrogenation theory and experimental studies on the de-hydrogenative polymerization of p-hydroxycinnamyl alcohols, is reviewed in this article.
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Lignins. Occurrence, Formation, Structure and Reactions
TL;DR: In this paper, a treatise on lignin sifts and knowledge accumulated from over a century of thought on nature's most enigmatic polymer and presents a workable, logical text.
Journal ArticleDOI
Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators.
TL;DR: Manganese oxidation by manganese peroxidase (MnP) was investigated, and no relationship between stimulation of enzyme activity and chelator size was found, suggesting that the substrate is free MnII rather than a MnII-chelator complex.