Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.
G. Hariharan,K. Prasannath +1 more
TLDR
A review of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi can be found in this article, where molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods.Abstract:
Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.read more
Citations
More filters
Journal ArticleDOI
Ten decadal advances in fungal biology leading towards human well-being
Ausana Mapook,Kevin D. Hyde,Khadija Nzingo Hassan,Blondelle M. Kemkuignou,Adéla Čmoková,Frank Surup,Eric Kuhnert,Pathompong Paomephan,Tianfan Cheng,Sybren de Hoog,Yinggai Song,Ruvishika S. Jayawardena,Abdullah M. S. Al-Hatmi,Tokameh Mahmoudi,Nadia Ponts,Lena Studt-Reinhold,Florence Richard-Forget,K. W. Thilini Chethana,Dulanjalee Harishchandra,Peter E. Mortimer,Huili Li,Saisamorn Lumyong,Worawoot Aiduang,Jaturong Kumla,Nakarin Suwannarach,Chitrabhanu S. Bhunjun,Feng-Ming Yu,Qi Zhao,Douglas Schaefer,Marc Stadler +29 more
TL;DR: In this paper , the authors highlight some important discoveries and developments in applied mycology and interdisciplinary Life Science research and highlight the importance of fungi as an understudied resource possessing huge potential for developing products that can greatly improve human well-being.
Journal ArticleDOI
Pathogens and predators impacting commercial production of microalgae and cyanobacteria
TL;DR: In this paper , a review of phytoplankton competitors, pathogens, and grazers of significance in commercial culture of microalgae and cyanobacteria is presented.
Journal ArticleDOI
Ascochyta rabiei: A threat to global chickpea production
TL;DR: Ascochyta rabiei infection and the emergence of new aggressive isolates directed the interest of researchers toward understanding the evolution of pathogenic determinants in this fungus as discussed by the authors .
Journal ArticleDOI
Comprehensive Review of Fungi on Coffee
Li-yuan Lu,Saowaluck Tibpromma,Samantha C. Karunarathna,Ruvishika S. Jayawardena,Saisamorn Lumyong,Jianchu Xu,Kevin D. Hyde +6 more
TL;DR: In this article , a comprehensive review of the fungal species reported on coffee based on taxonomy, life mode, host, affected plant part and region is presented and five major fungal diseases and mycotoxin-producing species (post-harvest diseases of coffee) are also discussed.
Journal ArticleDOI
One Health Probiotics as Biocontrol Agents: One Health Tomato Probiotics
TL;DR: In this paper , a new term for probiotics, "one health probiotics", beneficial for the unity of people, animals, and the environment was proposed. But, the proposed term was not applied to the tomato.
References
More filters
Journal ArticleDOI
Loop-mediated isothermal amplification of DNA
Tsugunori Notomi,Hiroto Okayama,Harumi Otawara-shi Masubuchi,Toshihiro Yonekawa,Keiko Watanabe,Nobuyuki Amino,Tetsu Hase +6 more
TL;DR: A novel method that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions that employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.
Journal ArticleDOI
Accelerated reaction by loop-mediated isothermal amplification using loop primers.
TL;DR: The LAMP method presented here uses loop primers to achieve reaction times of less than half that of the original LAMP, which should facilitate genetic analysis, including genetic diagnosis in the clinical laboratory.
Journal ArticleDOI
Review: A review of advanced techniques for detecting plant diseases
TL;DR: In this article, the authors present a review of the currently used technologies that can be used for developing a ground-based sensor system to assist in monitoring health and diseases in plants under field conditions.
Journal ArticleDOI
A window into third-generation sequencing
TL;DR: A new generation of single-molecule sequencing technologies (third-generation sequencing) that is emerging to fill this space, with the potential for dramatically longer read lengths, shorter time to result and lower overall cost.
Journal ArticleDOI
Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases
Yasuyoshi Mori,Tsugunori Notomi +1 more
TL;DR: The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future.