Recombination and Chromosome Segregation during the Single Division Meiosis in SPO12–1 and SPO13–1 Diploids
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A study of chromosome segregation and recombination during sporulation of spo12-1 and spo13-1 diploid strains of S. cerevisiae was performed in this paper.Abstract:
This paper reports a study of chromosome segregation and recombination during sporulation of spo12—1 and spo13–1 diploid strains of S. cerevisiae . These strains undergo a single division to form asci containing two diploid or near-diploid spores. The segregation of centromere-linked markers in the two-spored (dyad) products indicates that the division is generally equational. However, in a small percentage of the spo12–1 and spo13–1 cells, it appears that a meiosis I-like division occurs. Aberrant segregation of the MAT locus on chromosome III , yielding a monosomic and a trisomic spore pair, occurs in 12% of all dyads. The segregation patterns of markers at various distances from their centromeres and several pairs of markers on the same chromosome indicate that recombination takes place in both strains at nearly standard meiotic levels.read more
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Journal ArticleDOI
DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
TL;DR: DMC1 phenotypes provide further evidence that recombination and SC formation are interrelated processes and are consistent with a requirement for DNA-DNA interactions during SC formation, and additional evidence suggests that arrest occurs at a meiosis-specific cell cycle "checkpoint" in response to a primary defect in prophase chromosome metabolism.
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Disseminating the Genome: Joining, Resolving, and Separating Sister Chromatids During Mitosis and Meiosis
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Journal ArticleDOI
A central role for cohesins in sister chromatid cohesion, formation of axial elements, and recombination during yeast meiosis.
Franz Klein,Peter Mahr,Marta Galova,Sara B.C. Buonomo,Christine Michaelis,Knud Nairz,Kim Nasmyth +6 more
TL;DR: It is shown that Smc3p and a meiotic version of Scc1p called Rec8p are required for cohesion between sister chromatids, for formation of axial elements, for reciprocal recombination, and for preventing hyperresection of double-strand breaks during meiosis.
Journal ArticleDOI
A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae
TL;DR: In this article, the authors identify and analyze a meiotic reciprocal recombination hot spot in S. cerevisiae and find that double-strand breaks occur at two specific sites associated with the hot spot and that occurrence of these breaks depends upon meiotic recombination functions RAD50 and SPO11.
Book ChapterDOI
Mechanism and control of meiotic recombination initiation.
TL;DR: This chapter reviews the properties of Spo11 and the other gene products required for meiotic DSB formation in a number of organisms and discusses ways in which recombination initiation is coordinated with other events occurring in the meiotic cell.