Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
TLDR
In this paper, the base composition of deoxyribonucleic acid (DNA) was calculated by regression and correlation analysis and treated statistically by using only sets of data on DNA determined with the same strains.Abstract:
The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (ρ) or midpoint of thermal denaturation (Tm) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (Tm – 69.4). Tm appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (–1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between ρ and Tm was calculated from data of 197 strains; it agrees excellently with the above two equations.read more
Citations
More filters
Journal ArticleDOI
Bordetella avium sp. nov., Isolated from the Respiratory Tracts of Turkeys and Other Birds
TL;DR: The phenotypic and serological characteristics, together with the properties of the deoxyribon nucleic acid-ribosomal ribonucleic acid hybrids, indicated that this new species is a member of the genus Bordetella.
Journal ArticleDOI
Allorhizobium undicola gen. nov., sp. nov., nitrogen-fixing bacteria that efficiently nodulate Neptunia natans in Senegal
P. de Lajudie,E Laurent-Fulele,Anne Willems,U Torck,Renata Coopman,Matthew D. Collins,Karel Kersters,Bernard Dreyfus,Monique Gillis +8 more
TL;DR: A group of nodule isolates from Neptunia natans, an indigenous stemnodulated tropical legume found in waterlogged areas of Senegal, was studied and it was demonstrated that this group is phenotypically and phylogenetically separate from the known species.
Journal ArticleDOI
Isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in Beijing region
TL;DR: This research aims to isolate and identify nitrogen‐fixing bacilli from the plant rhizospheres in Beijing region of China.
Journal ArticleDOI
Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts.
J Vandenberghe,Ludo Verdonck,Rocio Robles-Arozarena,Gabriel Rivera,Annick Bolland,Marcos Balladares,Bruno Gomez-Gil,Jorge Calderón,Patrick Sorgeloos,Jean Swings +9 more
TL;DR: In Ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been determined, and the results suggest that putative probiotic and pathogenic strains each have specific genotypes.
Journal ArticleDOI
Isolation of bacterial DNA from soil.
TL;DR: A modification of Marmur's method was used to isolate DNA, but difficulties in separating DNA from humic substances gave low yields and impure DNA.
References
More filters
Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J. Marmur,Paul Doty +1 more
TL;DR: The previously discovered linear relation between the base composition of DNA, expressed in percentage of guanine plus cytosine bases, and the denaturation temperature, T m, has been further investigated and it appears that the measurement of the T m is a satisfactory means of determining base composition in DNA.
Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Journal ArticleDOI
The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine.
G. R. Wyatt,Seymour S. Cohen +1 more
Journal ArticleDOI
Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes.
Monica Riley,Barbara Maling +1 more
TL;DR: The DNA homopolymers dA and dT have been prepared enzymically using Escherichia coli DNA polymerase, and their properties have been studied, but the homopolymer pair dA:rU is not stable under any conditions of temperature and salt concentration tested.