Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
TLDR
In this paper, the base composition of deoxyribonucleic acid (DNA) was calculated by regression and correlation analysis and treated statistically by using only sets of data on DNA determined with the same strains.Abstract:
The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (ρ) or midpoint of thermal denaturation (Tm) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (Tm – 69.4). Tm appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (–1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between ρ and Tm was calculated from data of 197 strains; it agrees excellently with the above two equations.read more
Citations
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Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography
TL;DR: High-performance liquid chromatography is a promising alternative for determining the G+C content of bacterial deoxyribonucleic acid (DNA) and may also be more accurate than indirect methods, such as the buoyant density and thermal denaturation methods.
Journal ArticleDOI
DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
Johan Goris,Konstantinos T. Konstantinidis,Joel A. Klappenbach,Tom Coenye,Peter Vandamme,James M. Tiedje +5 more
TL;DR: It is concluded that ANI can accurately replace DDH values for strains for which genome sequences are available and reveal extensive gene diversity within the current concept of "species".
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Determination of DNA base composition by reversed-phase high-performance liquid chromatography
Jin Tamaoka,Kazuo Komagata +1 more
TL;DR: The system described here gives a direct and precise method for determining DNA base composition by reversed-phase high-performance liquid chromatography (HPLC).
Journal ArticleDOI
Studies on the spectrophotometric determination of DNA hybridization from renaturation rates
TL;DR: The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined and the agreement is excellent above a degree of binding of 25-30%.
Journal ArticleDOI
High diversity in DNA of soil bacteria.
TL;DR: The results show that the major part of DNA isolated from the bacterial fraction of soil is very heterogeneous, with a C0t1/2 value corresponding to about 4,000 completely different genomes of standard soil bacteria.
References
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Journal ArticleDOI
Deoxyribonucleic acid base composition in certain bacteria and their mutants with impaired respiration.
TL;DR: Deoxyribonucleic Acid Base Composition in Certain Bacteria and Their Mutants with Impaired Respiration and their mutants with Implantable Respiration are studied.
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Changes in the content and distribution of the nucleic acid bases in Chlorella during the life cycle.
Tatsuichi Iwamura,Jack Myers +1 more
TL;DR: In this article, changes in content and distribution of nitrogenous bases accompanying the life cycle of Chlorella ellipsoidea were examined by fractionation of cell materials into acid-soluble, ribonucleic acid (RNA), and deoxyribonucleically acid (DNA) fractions.
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Base composition of the deoxyribonucleic acid of sulfate-reducing bacteria
TL;DR: The results of these studies show that this group of microorganisms includes at least four subgroups characterized by significantly different values of the adenine plus thymine to guanine plus cytosine ratio.
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Adansonian Analysis and Deoxyribonucleic Acid Base Composition of Serratia marcescens
TL;DR: It was concluded that there is only a single species in the genus Serratia marcescens, and the variety designation kiliensis does not appear to be valid, since no subspecies clustering of strains with negative Voges-Proskauer reactions could be detected.