Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
TLDR
In this paper, the base composition of deoxyribonucleic acid (DNA) was calculated by regression and correlation analysis and treated statistically by using only sets of data on DNA determined with the same strains.Abstract:
The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (ρ) or midpoint of thermal denaturation (Tm) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (Tm – 69.4). Tm appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (–1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between ρ and Tm was calculated from data of 197 strains; it agrees excellently with the above two equations.read more
Citations
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Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography
TL;DR: High-performance liquid chromatography is a promising alternative for determining the G+C content of bacterial deoxyribonucleic acid (DNA) and may also be more accurate than indirect methods, such as the buoyant density and thermal denaturation methods.
Journal ArticleDOI
DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
Johan Goris,Konstantinos T. Konstantinidis,Joel A. Klappenbach,Tom Coenye,Peter Vandamme,James M. Tiedje +5 more
TL;DR: It is concluded that ANI can accurately replace DDH values for strains for which genome sequences are available and reveal extensive gene diversity within the current concept of "species".
Journal ArticleDOI
Determination of DNA base composition by reversed-phase high-performance liquid chromatography
Jin Tamaoka,Kazuo Komagata +1 more
TL;DR: The system described here gives a direct and precise method for determining DNA base composition by reversed-phase high-performance liquid chromatography (HPLC).
Journal ArticleDOI
Studies on the spectrophotometric determination of DNA hybridization from renaturation rates
TL;DR: The optical method of De Ley et al. (1970) for determining DNA/DNA homologies was reexamined and the agreement is excellent above a degree of binding of 25-30%.
Journal ArticleDOI
High diversity in DNA of soil bacteria.
TL;DR: The results show that the major part of DNA isolated from the bacterial fraction of soil is very heterogeneous, with a C0t1/2 value corresponding to about 4,000 completely different genomes of standard soil bacteria.
References
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Journal ArticleDOI
Variation and heterogeneity of base composition of deoxyribonucleic acids: A compilation of old and new data
TL;DR: A compilation of the previously accumulated data as well as some new results on the base composition of DNA from various organisms reveals some general features of its variation and heterogeneity.
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The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdg
A.D. Kaiser,David S. Hogness +1 more
TL;DR: Deoxyribonucleic acid of λ dg, isolated by phenol extraction, will transform galactose-negative strains of Escherichia coli K12, and the transforming agent appears to be the entire λdg chromosome since the phage genes c and mi are also present in the galactosed transformants.
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Isolation of high molecular weight DNA from Hemophilus influenzae
Kenneth I. Berns,C.A. Thomas +1 more
TL;DR: The nuclear DNA complement of Hemophilus influenzae has been determined to be 700 to 800 × 10 6 daltons and linkage of previously unlinked markers has been obtained in transformation assays.
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Biochemical studies on adenovirus multiplication: IV. Isolation, purification, and chemical analysis of adenovirus☆
Maurice Green,Magdalena Piña +1 more
TL;DR: Type 4 adenovirus prepared by the above procedure had a similar chemical composition and DNA base ratios as type 2 adenova, and evidence for the purity of the virus and for the double-stranded nature of viral DNA is discussed.
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Studies on polynucleotides
TL;DR: In the in vitro amino acid incorporation experiments, however, no polyaspartate synthesis has so far been detected in the presence of poly AAG, and the new codon sequences ApApG for lysine and GpApA for glutamic acid are proposed.