Reexamination of the Association Between Melting Point, Buoyant Density, and Chemical Base Composition of Deoxyribonucleic Acid
TLDR
In this paper, the base composition of deoxyribonucleic acid (DNA) was calculated by regression and correlation analysis and treated statistically by using only sets of data on DNA determined with the same strains.Abstract:
The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (ρ) or midpoint of thermal denaturation (Tm) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (Tm – 69.4). Tm appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (–1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between ρ and Tm was calculated from data of 197 strains; it agrees excellently with the above two equations.read more
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References
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Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J. Marmur,Paul Doty +1 more
TL;DR: The previously discovered linear relation between the base composition of DNA, expressed in percentage of guanine plus cytosine bases, and the denaturation temperature, T m, has been further investigated and it appears that the measurement of the T m is a satisfactory means of determining base composition in DNA.
Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
TL;DR: A comprehensive study of the buoyant density of DNA as a function of composition has been made and the linear relation previously reported has been confirmed.
Journal ArticleDOI
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Journal ArticleDOI
Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes.
Monica Riley,Barbara Maling +1 more
TL;DR: The DNA homopolymers dA and dT have been prepared enzymically using Escherichia coli DNA polymerase, and their properties have been studied, but the homopolymer pair dA:rU is not stable under any conditions of temperature and salt concentration tested.