Regulation of rat liver fructose 2,6-bisphosphatase.
TLDR
The findings suggest that this enzyme has both phosphohydrolase and phosphotransferase activities i.e. that it is bifunctional, and that both activities can be regulated by cAMP-dependent phosphorylation.About:
This article is published in Journal of Biological Chemistry.The article was published on 1982-07-10 and is currently open access. It has received 145 citations till now. The article focuses on the topics: Fructose 1,6-bisphosphatase & Fructolysis.read more
Citations
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Journal ArticleDOI
The protein phosphatases involved in cellular regulation
Nicholas K. Tonks,Philip Cohen +1 more
TL;DR: Spermine increased the activity of protein phosphatase-2A towards eight of nine substrates tested and was a more potent activator than spermidine, while putrescine had only a small effect, and Mn2+ could not mimic the activating effect of spermine on dephosphorylation of glycogen synthase (labelled in sites-3) by protein phosph atase 1.
Journal ArticleDOI
A mechanism for memory storage insensitive to molecular turnover: a bistable autophosphorylating kinase.
TL;DR: It is shown that a kinase that is activated by phosphorylation and an associated phosphatase form a bistable chemical switch that can be turned on by an external stimulus and that is not reset by protein turnover.
Journal ArticleDOI
6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis
TL;DR: A PFK-2/FBPase-2 sequence in the genome of one prokaryote could be the result of horizontal gene transfer from a eukaryote distantly related to all other organisms, possibly a protist.
Journal ArticleDOI
Glucagon physiology and pathophysiology in the light of new advances.
TL;DR: It now appears that α cells can respond directly to hyperglycaemia in the absence of insulin and β cells, but that antecedent hyperglyCAemia masks or attenuates this response.
Book
Antigen Retrieval Technique
TL;DR: Development of the antigen retrieval (AR) technique, a simple method of boiling archival paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry (IHC), was the frui...
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase.
TL;DR: The low-molecular-weight stimulator of phosphofructokinase has been purified from rat liver and is tentatively identified as fructose 2,6-bisphosphate.
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Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate
TL;DR: Fructose 2,6-bisphosphate, a known powerful stimulator of phosphofructokinase, was found to inhibit, at micromolar concentrations, liver and muscle fructose-1-6-biphosphate (D-fructose-1, 6-bisPhosphate 1-phosphohydrolase, EC 3.3.1.11).
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Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate.
TL;DR: Rat liver fructose-1,6-bisph phosphatase, which was assayed by measuring the release of 32P from fructose 1,6-[1-32P]bisphosphate at pH 7.5, exhibited hyperbolic kinetics with regard to its substrate.
Journal ArticleDOI
Fructose 2,6-bisphosphate. A new activator of phosphofructokinase.
TL;DR: Fructose 2,6-bisphosphate had properties identical with those of the activator purified from hepatocyte extracts and is likely that this new activator is an important physiologic factor of phosphofructokinase in vivo.