Journal ArticleDOI
Requirement for Xist in X chromosome inactivation
Graeme D. Penny,Graham F. Kay,Graham F. Kay,Steven A. Sheardown,Sohaila Rastan,Sohaila Rastan,Neil Brockdorff +6 more
TLDR
Evidence for gene targeting of Xist, the proposed candidate for the X inactivation centre, is provided, and its absolute requirement in the process of X chromosome inactivation is provided.Abstract:
The Xist gene has been proposed as a candidate for the X inactivation centre, the master regulatory switch locus that controls X chromosome inactivation So far this hypothesis has been supported solely by indirect evidence Here we describe gene targeting of Xist, and provide evidence for its absolute requirement in the process of X chromosome inactivationread more
Citations
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Long non-coding RNAs: new players in cell differentiation and development
Alessandro Fatica,Irene Bozzoni +1 more
TL;DR: The function of lncRNAs in developmental processes, such as in dosage compensation, genomic imprinting, cell differentiation and organogenesis, with a particular emphasis on mammalian development are described.
Journal ArticleDOI
lincRNAs: genomics, evolution, and mechanisms.
Igor Ulitsky,David P. Bartel +1 more
TL;DR: This Review outlines the emerging understanding of lincRNAs in vertebrate animals, with emphases on how they are being identified and current conclusions and questions regarding their genomics, evolution and mechanisms of action.
Journal ArticleDOI
Modular regulatory principles of large non-coding RNAs
TL;DR: This work synthesizes studies to provide an emerging model whereby large ncRNAs might achieve regulatory specificity through modularity, assembling diverse combinations of proteins and possibly RNA and DNA interactions.
Journal ArticleDOI
Chromatin modification and epigenetic reprogramming in mammalian development
TL;DR: The regulation of higher-order chromatin structures by DNA methylation and histone modification is crucial for genome reprogramming during early embryogenesis and gametogenesis, and for tissue-specific gene expression and global gene silencing.
Journal ArticleDOI
X-inactivation profile reveals extensive variability in X-linked gene expression in females
TL;DR: A comprehensive X-inactivation profile of the human X chromosome is presented, representing an estimated 95% of assayable genes in fibroblast-based test systems, and suggests a remarkable and previously unsuspected degree of expression heterogeneity among females.
References
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Journal ArticleDOI
Gene Action in the X -chromosome of the Mouse ( Mus musculus L.)
TL;DR: Ohno and Hauschka1 showed that in female mice one chromosome of mammary carcinoma cells and of normal diploid cells of the ovary, mammary gland and liver was heteropyKnotic and suggested that the so-called sex chromatin was composed of one heteropyknotic X-chromosome.
Journal ArticleDOI
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
TL;DR: A positive and negative selection procedure is described that enriches 2,000-fold for those cells that contain a targeted mutation in mouse embryo-derived stem cells.
Book
Teratocarcinomas and embryonic stem cells : a practical approach
TL;DR: The results allowed us to assess the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
Journal ArticleDOI
A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome
Carolyn J. Brown,Andrea Ballabio,James L. Rupert,Ronald G. Lafreniere,Markus Grompe,Rossana Tonlorenzi,Huntington F. Willard +6 more
TL;DR: This gene, called XIST (for Xi-specific transcripts), is a candidate for a gene either involved in or uniquely influenced by the process of X inactivation, and is described as an X-linked gene with a novel expression pattern.
Journal ArticleDOI
The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus.
Carolyn J. Brown,Brian Hendrich,Jim L. Rupert,Ronald G. Lafreniere,Yigong P. Xing,Jeanne B. Lawrence,Huntington F. Willard +6 more
TL;DR: Human XIST cDNAs containing at least eight exons and totaling 17 kb have been isolated and sequenced within the region on the X chromosome known to contain the X inactivation center, suggesting that XIST may function as a structural RNA within the nucleus.