Journal ArticleDOI
Reversible alkylation of a methionyl residue near the active site of β-galactosidase
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This article is published in Biochemistry.The article was published on 1972-08-15. It has received 103 citations till now. The article focuses on the topics: Residue (chemistry) & Active site.read more
Citations
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Book ChapterDOI
Glycoside Hydrolases: Mechanistic Information from Studies with Reversible and Irreversible Inhibitors
TL;DR: The results of the studies that permit some generalizations on the catalytic mechanism of glycoside hydrolases from widely differing sources and with different sugar and aglycon specificities and that have become available over the past 15 years are discussed.
Journal ArticleDOI
Methionine in proteins defends against oxidative stress
Shen Luo,Rodney L. Levine +1 more
TL;DR: The hypothesis that methionine in protein acts as an endogenous antioxidant in cells is supported, and one of the most abundant proteins in the cell, elongation factor Tu, was found to be more oxidatively modified in norleucine‐substituted cells, consistent with loss of the antioxidant defense provided by methionin residues.
Journal ArticleDOI
Editing of errors in selection of amino acids for protein synthesis.
TL;DR: The availability of X-ray crystallographic structures of some synthetases, combined with site-directed mutagenesis, allows insights into molecular details of the extraordinary selectivity of synthetase, including the editing function.
Patent
Artifical chromosomes, uses thereof and methods for preparing artificial chromosomes
Gyula Hadlaczky,Aladar A. Szalay +1 more
TL;DR: In this paper, the authors present methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes and methods for purification and insertion of heterologous DNA into artificial chromosomes.
Journal ArticleDOI
Covalent inhibitors of glycosidases and their applications in biochemistry and biology
TL;DR: Some of the compounds used to covalently modify glycosidases, their use in residue identification, structural and mechanistic investigations, and finally their applications, both in vitro and in vivo, to complex biological systems are discussed.
References
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Journal ArticleDOI
Esters of Methanesulfonic Acid as Irreversible Inhibitors of Acetylcholinesterase
Richard J. Kitz,Irwin B. Wilson +1 more
Journal ArticleDOI
Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12
Book ChapterDOI
1 Steady State Kinetics
TL;DR: This chapter discusses the kinetics of the steady state—that is, to conditions where the reactant concentrations and their Michaelis constants greatly exceed that of the catalyst or where there is a dynamic steady state with substrates being continually supplied and products continually removed.
Journal ArticleDOI
The reaction of iodoacetate with methionine.
TL;DR: In order to ascertain whether sulfonium salt formation might have taken place, the chemistry of the reaction of iodoacetate with methionine has been examined in more detail and Ion exchange chromatography has been used as a means of following the reaction.
Related Papers (5)
Identification of an essential carboxylate group at the active site of lacZ beta-galactosidase from Escherichia coli.
Monika Herrchen,Günter Legler +1 more