Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12
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This article is published in Journal of Biological Chemistry.The article was published on 1965-06-01 and is currently open access. It has received 609 citations till now. The article focuses on the topics: Galactosidases & Escherichia coli.read more
Citations
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes
TL;DR: It is shown how the method can be used to determine the approximate molecular weight of the DNA topoisomerase polypeptide by sectioning a gel on which a partially pure sample has been fractionated by electrophoresis.
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Labeling of proteins by reductive methylation using sodium cyanoborohydride.
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Gene structure and extracellular secretion of Neisseria gonorrhoeae IgA protease
TL;DR: A model in which the helper serves as a pore for excretion of the protease domain through the outer membrane of IgA protease acquires an active conformation as its extracellular transport proceeds and is released as a proform from the membrane-bound helper by autoproteolysis.
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Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining.
Eiji Ishikawa,Masayoshi Imagawa,Seiichi Hashida,Shinji Yoshitake,Yoshitaka Hamaguchi,Tetsuo Ueno +5 more
TL;DR: The use of an enzyme as a label has a number of advantages over the use of other labels in both immunohistochemistry and immunoassay, and antibody or antibody fragments labeled with enzymes of small molecular weight can more readily permeate cells of tissue sections than ferritin-labeled antibodies.
References
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Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
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Automatic recording apparatus for use in the chromatography of amino acids.
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Genetic regulatory mechanisms in the synthesis of proteins.
François Jacob,Jacques Monod +1 more
TL;DR: The synthesis of enzymes in bacteria follows a double genetic control, which appears to operate directly at the level of the synthesis by the gene of a shortlived intermediate, or messenger, which becomes associated with the ribosomes where protein synthesis takes place.
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A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
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