Journal ArticleDOI
Salt- and glyphosate-induced increase in glyoxalase I activity in cell lines of groundnut (Arachis hypogaea)
TLDR
Inhibition of cell division decreased glyoxalase I activity and vice versa, thus further corroborating its role as a cell division marker enzyme, as well as confirming its role to stress tolerance as well.Abstract:
Glyoxalase I (EC 4.4.1.5) activity has long been associated with rapid cell proliferation, but experimental evidence is forthcoming, linking its role to stress tolerance as well. Proliferative callus cultures of groundnut (Arachis hypogaea L. cv. JL24) showed a 3.3-fold increase in glyoxalase I activity during the logarithmic growth phase, correlating well with the data on FW gain and mitotic index. Inhibition of cell division decreased glyoxalase I activity and vice versa, thus further corroborating its role as a cell division marker enzyme. Cell lines of A. hypogaea selected in the presence of high salt (NaCl) and herbicide (glyphosate) concentrations, yielded 4.2- to 4.5-fold and 3.9- to 4.6-fold elevated glyoxalase I activity, respectively, in a dose dependent manner reflective of the level of stress tolerance. The stress-induced increase in enzyme activity was also accompanied by an increase in the glutathione content. Exogenous supplementation of glutathione could partially alleviate the growth inhibition of callus cultures induced by methylglyoxal and d-isoascorbic acid, but failed to recover the loss in glyoxalase I activity due to d-isoascorbic acid. The adaptive significance of elevated glyoxalase I activity in maintaining glutathione homeostasis has been discussed in view of our understanding on the role of glutathione in the integration of cellular processes with plant growth and development under stress conditions.read more
Citations
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Journal ArticleDOI
Reactive oxygen species homeostasis and signalling during drought and salinity stresses
TL;DR: An overview of ROS homeostasis and signalling in response to drought and salt stresses is provided and the current understanding of ROS involvement in stress sensing, stress signalling and regulation of acclimation responses is discussed.
Journal ArticleDOI
Glutathione as an Antioxidant and Regulatory Molecule in Plants Under Abiotic Stress Conditions
TL;DR: The size and redox state of the GSH pool is discussed, including their regulation, their role in redox signalling and defense processes, and the changes caused by abiotic stress.
Journal ArticleDOI
Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings
Mirza Hasanuzzaman,Mirza Hasanuzzaman,Mohammad Anwar Hossain,Mohammad Anwar Hossain,Masayuki Fujita +4 more
TL;DR: The results suggest that the exogenous application of NO rendered the plants more tolerant to salinity-induced oxidative damage by enhancing their antioxidant defense and MG detoxification systems.
Journal ArticleDOI
Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress
Mohammad Anwar Hossain,Mohammad Anwar Hossain,Mirza Hasanuzzaman,Mirza Hasanuzzaman,Masayuki Fujita +4 more
TL;DR: It is suggested that both betaine and proline provide a protective action against Cd-induced oxidative stress by reducing H2O2 and lipid peroxidation levels and by increasing the antioxidant defense and MG detoxification systems.
Journal ArticleDOI
Selenium Pretreatment Upregulates the Antioxidant Defense and Methylglyoxal Detoxification System and Confers Enhanced Tolerance to Drought Stress in Rapeseed Seedlings
TL;DR: The results indicate that the exogenous application of Se increased the tolerance of the plants to drought-induced oxidative damage by enhancing their antioxidant defense and methylglyoxal detoxification systems.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Glutathione homeostasis in plants: implications for environmental sensing and plant development
TL;DR: The aim of this review is to assess recent biochemical, molecular, genetic, and physiological advances which are increasing the understanding of the mechanisms by which plant glutathione homeostasis is controlled and the role of glutathion in the integration of cellular processes with plant growth and development under stress.
Journal ArticleDOI
The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 Gene Defines a Glutathione-Dependent Pathway Involved in Initiation and Maintenance of Cell Division during Postembryonic Root Development
Teva Vernoux,Robert C. Wilson,Kevin Andrew Seeley,Jean-Philippe Reichheld,Sandra E Muroy,Spencer Brown,Spencer C. Maughan,Christopher S. Cobbett,Marc Van Montagu,Dirk Inzé,Mike J. May,Zinmay Renee Sung +11 more
TL;DR: It is shown that the G1-to-S phase transition requires an adequate level of GSH, which suggests the existence of a GSH-dependent developmental pathway essential for initiation and maintenance of cell division during postembryonic root development.