Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms
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Citations
Non-scanning motionless fluorescence three-dimensional holographic microscopy
Quantitative Phase Imaging
Introduction to Modern Digital Holography: With Matlab
Superresolution digital holographic microscopy for three-dimensional samples
Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy
References
Introduction to Fourier Optics
Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
Phase-shifting digital holography
Digital holography for quantitative phase-contrast imaging.
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
Related Papers (5)
Non-scanning motionless fluorescence three-dimensional holographic microscopy
Frequently Asked Questions (13)
Q2. How can one reconstruct a hologram in a chosen axial plane of focus?
The reconstruction of the hologram in a chosen axial plane of focus z zR can be obtained by digital Fresnel back propagation from the hologram for a distance z0 zR.
Q3. How can one tile a synthetic pupil?
By adding coherently the reconstruction amplitudes of a number of holograms with different spatial frequency shifts, one can, in principle, tile a synthetic pupil with an area far exceeding the pupil disk of the objective.
Q4. What is the effect of the holographic interferences?
Combining the complex amplitudes of the three off-axis holograms leads to destructive interferences at the rim of the main lobe of the Airy disc, leading to a narrowing of its size.
Q5. What is the expectation of the reconstruction of the on-axis hologram?
The expectation is thatthe reconstruction of the on-axis hologram will show, at best, barely unresolved beads, while the composite reconstruction should reveal beads that are resolved.
Q6. How can a single-sideband hologram be obtained?
A single-sideband hologram can be obtained from this data in two ways: either by heterodyne detection using a frequency difference between the two pupils,45 or by homodyne detection46 using three scans with three fixed phase differences between the two pupils.
Q7. Where is J. Rosen currently on leave?
J. Rosen is also with the Department of Electrical and Computer Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 84105, Israel, from which he is presently on leave.
Q8. How much resolution can be achieved by introducing off-axis FZP?
If the off-axis FZP are introduced on the specimen through the objective, as done in the experiment, the authors can expect a resolution limit down to a factor 0.5 that of the objective.
Q9. How can one tile an object’s spatial frequency spectrum?
By combining several holograms with shifts in different angular directions, it is thus possible to tile an object’s spatial frequency spectrum that extends, in principle, far beyond the objective’s pupil disk.
Q10. What is the funding source for this research?
This research was funded in part by a grant from the National Institute of Health, Office of Extramural Research, grant R21 RR018440, and by a grant from the National Science Foundation, grant DBI0420382.
Q11. What is the hologram's radius of curvature?
Using the relation F a2 EXz0 with an excitation wavelength EX 532 nm, the radius of curvature of the hologram is found to be z0 50 m, and its equivalent NA is a z0 0.35.
Q12. How can one record an off-axis hologram?
To extend the spectrum beyond the objective’s cutoff in a particular direction specified by a unit vector n̂, one can record an off-axis hologram by scanning an off-axis FZP on the specimen.
Q13. What is the hologram constructed line by line after bandpass filtering?
The hologram is constructed line by line after bandpass filtering at the modulation frequency, as previously described, and reconstructed digitally using MATLAB codes (MATLAB 7.1.0.144 R14 SP3) on a standard personal computer (PC).