Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants.
Reads0
Chats0
TLDR
Deletion derivatives of the maize transposable element Activator were constructed in vitro and inserted into a kanamycin resistance gene and it is shown that although similar lengths of DNA are required at each terminus, the termini are not interchangeable.Abstract:
Deletion derivatives of the maize transposable element Activator (Ac) were constructed in vitro and inserted into a kanamycin resistance gene. These constructions were then introduced into tobacco protoplasts derived from plants previously transformed with Ac. The ability of each deletion derivative to excise was measured by whether or not kanamycin-resistant tobacco calli were recovered. This allowed us to determine the length of DNA present at each terminus that is required to respond to the products expressed by the Ac element present in the genome. We show that around 200 base pairs (bp) are required at both ends for excision to occur at wild-type levels. When between 100 and 200 bp were retained at one of the ends, reduced frequencies of excision were detected. With less than 100 bp remaining at either end, no excision was detected. In addition, we show that although similar lengths of DNA are required at each terminus, the termini are not interchangeable. The significance of these data is discussed with respect to the protein(s) which interact(s) with the termini of Ac.read more
Citations
More filters
Journal ArticleDOI
Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements.
Venkatesan Sundaresan,Patricia Springer,Tom Volpe,Samuel Haward,Samuel Haward,Jonathan D. G. Jones,Caroline Dean,Hong Ma,Robert A. Martienssen +8 more
TL;DR: The development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development and suggests that the identification and cloning ofArabidopsis genes expressed in any developmental process is feasible using this system.
Journal ArticleDOI
Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition.
TL;DR: The identification of the minimal cis-sequence of the Tol2 element and the construction of mini-Tol2 vectors will facilitate development of useful transposon tools in vertebrates and established a basis for further biochemical and molecular biological studies for understanding roles of the repetitive sequence in the subterminal region in transposition.
Book
Homologous Recombination and Gene Silencing in Plants
TL;DR: This work has shown that post-transcriptional inhibition of gene expression in transgenic plants is an autoregulatory model and Mechanisms for maintaining genome size and generating genomic diversity in geminiviruses are investigated.
Book ChapterDOI
The hAT and CACTA Superfamilies of Plant Transposons
Reinhard Kunze,Clifford F. Weil +1 more
TL;DR: This chapter focuses primarily on what is known about the hAT and Enhancer/Suppressor-mutator (En/Spm) superfamilies of plant mobile elements and features of their biology and their transposition mechanisms that have become known in the past decade.
Journal ArticleDOI
Abortive gap repair : underlying mechanism for ds element formation
Eitan Rubin,Avraham A. Levy +1 more
TL;DR: The data presented here suggest that abortive Ac-induced gap repair, through the synthesis-dependent strand-annealing pathway, is the underlying mechanism for Ds element formation.
References
More filters
Journal ArticleDOI
Ac transposition in transgenic tomato plants
TL;DR: Southern analysis of leaf tissue indicated that in nine out of eleven transgenic plants, Ac was excised from the T-DNA and reintegrated into new chromosomal locations, suggesting transposition during later stages of transgenic plant development.