Journal ArticleDOI
Sparse matrix sampling: a screening method for crystallization of proteins
Jaru Jancarik,Sung-Hou Kim +1 more
TLDR
A set of screening conditions for initial experiments in protein crystallization has been developed, tested, and is herein presented as discussed by the authors, which are empirically derived based on known or published crystallization conditions of various proteins in the past, so as to sample as large a range of buffer, pH, additive and precipitant variables as possible, using small amounts of proteins.Abstract:
A set of screening conditions for initial experiments in protein crystallization has been developed, tested, and is herein presented. These solution and precipitant conditions are empirically derived based on known or published crystallization conditions of various proteins in the past, so as to sample as large a range of buffer, pH, additive and precipitant variables as possible, using small amounts of proteins. The 50 crystallization conditions have been tested on 15 previously crystallized proteins, all of which were also crystallized in at least one form by this screen. This method is also shown to be highly successful in the crystallization of proteins which had not previously been crystallized.read more
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Journal ArticleDOI
Liquid phase condensation in cell physiology and disease.
TL;DR: The findings together suggest that several membrane-less organelles have been shown to exhibit a concentration threshold for assembly, a hallmark of phase separation, and represent liquid-phase condensates, which form via a biologically regulated (liquid-liquid) phase separation process.
Journal ArticleDOI
Crystal structure of opsin in its G-protein-interacting conformation
Patrick Scheerer,Jung Hee Park,Peter W. Hildebrand,Yong Ju Kim,Norbert Krauss,Hui-Woog Choe,Hui-Woog Choe,Klaus Peter Hofmann,Klaus Peter Hofmann,Oliver P. Ernst +9 more
TL;DR: The 3.2 Å crystal structure of the bovine Ops*–GαCT peptide complex is presented and signal transfer from the receptor to the G protein nucleotide-binding site is discussed.
Journal ArticleDOI
Crystal structure of the ligand-free G-protein-coupled receptor opsin
Jung Hee Park,Patrick Scheerer,Klaus Peter Hofmann,Klaus Peter Hofmann,Hui-Woog Choe,Hui-Woog Choe,Oliver P. Ernst +6 more
TL;DR: The crystal structure of ligand-free native opsin from bovine retinal rod cells is presented and shows prominent structural changes in the conserved E(D)RY and NPxxY(x)5,6F regions and in TM5–TM7.
Journal ArticleDOI
Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases.
TL;DR: The crystal structure of the catalytically active core domain of HIV-1 integrase was determined and it is revealed that this domain of integrase belongs to a superfamily of polynucleotidyl transferases that includes ribonuclease H and the Holliday junction resolvase RuvC.
Journal ArticleDOI
Three-dimensional structure of the β subunit of E. coli DNA polymerase III holoenzyme: A sliding DNA clamp
TL;DR: A potential structural relationship is suggested between the beta subunit and proliferating cell nuclear antigen (PCNA, the eukaryotic polymerase delta [and epsilon] processivity factor), and the gene 45 protein of the bacteriophage T4 DNA polymerase.
References
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Book
Preparation and analysis of protein crystals
TL;DR: A complete guide to techniques and procedures for the preparation of proteins for crystallographic studies is given in this article, which describes methods for protein crystallization; formation of isomorphous heavy atoms; x-ray diffraction and analysis; and photographic and computer-based data collection methods and instrumentation.
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