Journal ArticleDOI
Spectrophotometric evaluation of interferences in three iron reactions for the determination of serum total cholesterol
TLDR
In this paper, a spectrophotometric and chemical evaluation of reported interferences for three iron reactions for the determinations of serum cholesterol has been presented, and it has been shown that all three reactions are affected by various interfering substances, such as 2-thiouracil, nitrate, azide, bromide, diethylstilbesterol, and steroids.About:
This article is published in Microchemical Journal.The article was published on 1978-06-01. It has received 5 citations till now. The article focuses on the topics: Ferric & Reagent.read more
Citations
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Journal ArticleDOI
Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules.
TL;DR: Current progress in modifying enzyme immobilized biosensors to detect biologically essential molecules (glucose, urea, cholesterol) is covered and a discussion of the different types of enzyme immobilization is provided.
Journal ArticleDOI
Cholesterol methodology for human studies
TL;DR: A classification and review of the methodology involved in the determination of serum cholesterol for human (or animal) studies are presented in this article, with the purpose of both is to enable selection of a technique appropriate for the assay intended with a reasonable understanding of its advantages, disadvantages and limitations.
Book ChapterDOI
Cholesterol and Related Sterols: Roles in Membrane Structure and Function
TL;DR: In this paper, a new model for the required roles of cholesterol in mammalian cell biology was proposed, where cholesterol decreases membrane permeability through an ordering of the lipid hydrocarbon chains.
Journal ArticleDOI
A review of reactions of some sterols in strongly acidic media
TL;DR: In this paper, the dependence of the rate of generation of a chromogen on acidity was studied and the optimum acidity and reaction time was determined for individual sterols, including estrogens and related steroids with an aromatic ring A and a hydroxy group on ring D.
References
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Journal ArticleDOI
A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity.
TL;DR: The simplified but precise method described in this paper involves treatment of the serum with alcoholic potassium hydroxide to liberate the cholesterol from the lipoprotein complexes and to saponify the cholesterol esters.
Journal ArticleDOI
A new method for the direct determination of serum cholesterol.
TL;DR: Precision and reproducibility are demonstrated as well as the absorption characteristics of the purple color produced through the spectral range of 400 to 700 mμ.
Journal ArticleDOI
Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents
Amritlal C. Parekh,David H. Jung +1 more
Journal ArticleDOI
Direct Manual Determination of Serum Total Cholesterol with a Single Stable Reagent
TL;DR: A simple, direct, and specific manual method for quantitative determination of total cholesterol in serum, which requires no extraction of the serum, and a single stable reagent and 50 µl of sample is used.
Related Papers (5)
Modification of the ferric chloride assay for the spectrophotometric determination of ferric and total iron in acidic solutions containing high concentrations of copper
Interference of sodium azide with measurement of serum uric acid by the direct acid ferric reduction procedure.
T Z Liu,H Khayam-Bashi +1 more