Stable Overexpression of Manganese Superoxide Dismutase in Mitochondria Identifies Hydrogen Peroxide as a Major Oxidant in the AP-1-mediated Induction of Matrix-degrading Metalloprotease-1
Jutta Wenk,Peter Brenneisen,Meinhard Wlaschek,Arndt Poswig,Karlis Briviba,Terry D. Oberley,Karin Scharffetter-Kochanek +6 more
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It is found that enhanced Mn-SOD activity, via an unbalanced H2O2overproduction and detoxification, induces MMP-1 mRNA levels, and this effect is at least partly mediated by the DNA recognition sequence AP-1.About:
This article is published in Journal of Biological Chemistry.The article was published on 1999-09-03 and is currently open access. It has received 226 citations till now. The article focuses on the topics: Reactive oxygen species & Superoxide.read more
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Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple.
TL;DR: Estimates can be used to more fully understand the redox biochemistry that results from oxidative stress, which hopefully will provide a rationale and understanding of the cellular mechanisms associated with cell growth and development, signaling, and reductive or oxidative stress.
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Reactive oxygen species in cancer
Geou Yarh Liou,Peter Storz +1 more
TL;DR: The generation of ROS within tumour cells, their detoxification, their cellular effects, as well as the major signalling cascades they utilize are discussed, but also an outlook on their modulation in therapeutics is provided.
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PLANT MITOCHONDRIA AND OXIDATIVE STRESS: Electron Transport, NADPH Turnover, and Metabolism of Reactive Oxygen Species.
TL;DR: The alternative oxidase and possibly NDin(NADH) function to limit mitochondrial ROS production by keeping the ETC relatively oxidized, together with small antioxidants such as glutathione, that help remove ROS.
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Reactive oxygen species in tumor progression.
TL;DR: The finding that a diet rich in antioxidants or the elimination of ROS by antioxidant compounds prevents the development of certain cancers provided the setting for subsequent investigation of the tumorigenic actions of reactive oxygen species.
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Mitochondrial generation of superoxide and hydrogen peroxide as the source of mitochondrial redox signaling
TL;DR: This review examines the generation of reactive oxygen species by mammalian mitochondria, and the status of different sites of production in redox signaling and pathology, and identifies specific suppressors of two sites that allow the cellular roles of mitochondrial superoxide/hydrogen peroxide production to be investigated without catastrophic confounding bioenergetic effects.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Book ChapterDOI
Catalase in vitro
TL;DR: In this article, the catalytic activity of catalase has been investigated using ultraviolet (UV) spectrophotometry and Titrimetric methods, which is suitable for comparative studies for large series of measurements.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.
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A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
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Oxidative stress: oxidants and antioxidants
TL;DR: These low molecular mass antioxidant molecules add significantly to the defense provided by the enzymes superoxide dismutase, catalase and glutathione peroxidases, which are termed ‘oxidative stress’.