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Journal ArticleDOI

Structural and functional features of different types of cytoplasmic fatty acid-binding proteins

04 Jan 1991-Biochimica et Biophysica Acta (Elsevier)-Vol. 1081, Iss: 1, pp 1-24
TL;DR: Article de synthese sur les donnees recentes de caracteristiques structurales et physicochimiques de divers types of proteines de liaison aux acides gras, avec la signification physiologique de ces diversites.
About: This article is published in Biochimica et Biophysica Acta.The article was published on 1991-01-04. It has received 366 citations till now.
Citations
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Journal ArticleDOI
TL;DR: A review of recent findings on the diversity of FABPs and their proposed roles in fatty acid (FA) metabolism and other cellular processes focuses on mammalian FABP types.
Abstract: Fatty acid-binding proteins (FABPs) are members of a superfamily of lipid-binding proteins, and occur intracellularly in vertebrates and invertebrates. This review presents recent findings on the diversity of these FABPs and their proposed roles in fatty acid (FA) metabolism and other cellular processes. Special attention is paid to the structural features of the different mammalian FABP types and the physiological role of these proteins in FA transport, cell growth and differentiation, cellular signalling, gene transcription and cytoprotection. Additionally, data on FABP knockout mice and the implication of FABP in medicine are discussed.

474 citations

Book ChapterDOI
TL;DR: This chapter focuses on the structural analyses and comparisons between members of a multigene family of hydrophobic ligand-binding proteins and provides a detailed comparison of intra- and extracellular lipid binding proteins with known crystal structures.
Abstract: Publisher Summary This chapter focuses on the structural analyses and comparisons between members of a multigene family of hydrophobic ligand-binding proteins. It discusses the structural motif, general characteristics of the binding cavity, ligand entry, and the portal hypothesis and provides a detailed comparison of intra- and extracellular lipid binding proteins with known crystal structures. The members of this family are referred as lipid-binding proteins (LBPs). This collection of proteins can be subdivided into two groups: the intracellular lipid-binding protein family (iLBP) and the extracellular lipid binding protein family (eLBP). The comparison primarily deals with the iLBP branch because this family is becoming structurally well characterized. However, the structural comparisons are extended to some members of the eLBP family because the basic structural motif used to bind hydrophobic ligands applies to both. The products of hydrolysis of the intestinal lipids, including fatty acids, cholesterol, monoglycerides, and lysophospholipids, have very low solubilities and are absorbed by biliary micelles in the gut. These micelles diffuse through the glycocalyx, which stabilizes an unstirred water layer at the surface of the enterocyte. The chapter concludes with a discussion of the results of site-directed mutagenesis studies, the thermodynamics of lipid binding, and considerations of protein stability and folding.

466 citations

Journal ArticleDOI
TL;DR: The partially overlapping expression pattern with that of cellular retinoid binding proteins suggests that B-FABP is involved in the metabolism of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development.
Abstract: Fatty acid binding proteins (FABPs) are a multigene family of small intracellular proteins that bind hydrophobic ligands. In this report we describe the cloning and expression pattern of a novel member of this gene family that is specifically expressed in the developing and adult nervous system and thus was designated brain (B)-FABP. B-FABP is closely related to heart (H)-FABP with 67% amino acid identity. B-FABP expression was first detected at mouse embryonic day 10 in neuroepithelial cells and its pattern correlates with early neuronal differentiation. Upon further development, B-FABP was confined to radial glial cells and immature astrocytes. B-FABP mRNA and protein were found in glial cells of the peripheral nervous system such as satellite cells of spinal and cranial ganglia and ensheathing cells of the olfactory nerve layer from as early as embryonic day 11 until adulthood. In the adult mouse brain, B-FABP was found in the glia limitans, in radial glial cells of the hippocampal dentate gyrus and Bergman glial cells. These findings suggest a function of B-FABP during neurogenesis or neuronal migration in the developing nervous system. The partially overlapping expression pattern with that of cellular retinoid binding proteins suggests that B-FABP is involved in the metabolism of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development.

350 citations


Cites background from "Structural and functional features ..."

  • ...FABPs, in general, are synthesised in specific differentiated tissues (Matarese et al., 1989; Veerkamp et al., 1991)....

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References
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Journal ArticleDOI
01 Jul 1988-Science
TL;DR: Phylogenetic mapping of the conserved protein kinase catalytic domains can serve as a useful first step in the functional characterization of these newly identified family members.
Abstract: In recent years, members of the protein kinase family have been discovered at an accelerated pace. Most were first described, not through the traditional biochemical approach of protein purification and enzyme assay, but as putative protein kinase amino acid sequences deduced from the nucleotide sequences of molecularly cloned genes or complementary DNAs. Phylogenetic mapping of the conserved protein kinase catalytic domains can serve as a useful first step in the functional characterization of these newly identified family members.

4,838 citations

Journal ArticleDOI
10 Feb 1989-Cell
TL;DR: The location, orientation, and structure of the hormone regulatory elements (HRE) in nine hormonally modulated genes is described and a model for the interaction is proposed in which a dimer of the receptor in head-to-head orientation binds to the inverted symmetry element of the HRE.

3,331 citations

Journal ArticleDOI
01 Dec 1987-Nature
TL;DR: Analysis of complementary DNA encoding a novel gene product reveals striking similarity to the steroid and thyroid hormone receptors and Binding and transcription activational studies show it to be a receptor for the vitamin A-related morphogen retinoic acid.
Abstract: Analysis of complementary DNA encoding a novel gene product reveals striking similarity to the steroid and thyroid hormone receptors. Binding and transcription activational studies show it to be a receptor for the vitamin A-related morphogen retinoic acid.

1,952 citations

Journal ArticleDOI
TL;DR: A review of the available information about fatty acid binding to plasma albumin is presented, and stepwise association constants for binding to human albumin of fatty acids containing 6-18 carbon atoms indicate that each mole of fatty acid binds with a different affinity.

915 citations

Journal ArticleDOI
01 Nov 1986-Nature
TL;DR: A possible binding site for retinol in BLG has been identified by model-building and a role for BLG in vitamin A transport is suggested and specific receptors for the BLG–retinol complex in the intestine of neonate calves are discovered.
Abstract: Since its first isolation1, bovine β-lactoglobulin (BLG) has been an enigma: although it is abundant in the whey fraction of milk, its function is still not clear. The results of the many physicochemical studies on the protein need a structural interpretation. We report here the structure of the orthorhombic crystal form of cow BLG at pH 7.6, at a resolution of 2.8 A. It has an unusual protein fold, composed of two slabs of antiparallel β-sheet, which shows a remarkable similarity to plasma retinol-binding protein. A possible binding site for retinol in BLG has been identified by model-building. This suggests a role for BLG in vitamin A transport and we have discovered specific receptors for the BLG–retinol complex in the intestine of neonate calves.

865 citations