Journal ArticleDOI
Structure and function of informofers.
TLDR
DNA-like RNA (dRNA) is found in the nucleus as a complex with globular protein particles called informofers, and can form complexes in vitro and in vivo with different kinds of dRNA, including virus-specific RNA synthesized in adenovirus-infected cells.Abstract:
DNA-like RNA (dRNA) is found in the nucleus as a complex with globular protein particles called informofers. Intact informofers may be detached from the dRNA and can form complexes in vitro and in vivo with different kinds of dRNA, including virus-specific RNA synthesized in adenovirus-infected cells.read more
Citations
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Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
TL;DR: It is proposed that the lamina is a biological polymer which can undergo reversible disassembly during mitosis, and are not structural elements of an internal "nuclear protein matrix".
Journal ArticleDOI
Identification and characterization of the packaging proteins of core 40S hnRNP particles
TL;DR: The similarity in protein composition of core RNP particles from different cell types is consistent with a conserved particle structure and function in eucaryotes.
Journal ArticleDOI
Structure and Function of Nuclear and Cytoplasmic Ribonucleoprotein Particles
TL;DR: Evidence for, and Isolation of, hnRNP Particles is found, and general Structural Features of hNUCLEAR RIBONUCLEOPROTEIN Particles are found.
Journal ArticleDOI
Proteins associated with heterogeneous nuclear RNA in eukaryotic cells.
TL;DR: It is proposed that this cell-specificity reflects a situation in which HnRNA molecules that differ in nucleotide sequence are complexed with different sets of proteins, so that the resulting HnRNP particles are biochemically distinct at each genetic locus.
Journal ArticleDOI
Specific protection from phenocopy induction by heat shock
TL;DR: The results suggest that the protection for survival and against phenocopy induction is due to storage of messenger RNA.
References
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Journal ArticleDOI
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane
David Gillespie,Sol Spiegelman +1 more
TL;DR: An improved method for the formation of DNA—RNA hybrids is described, quantitatively more certain than annealing in liquid, since the competing DNA renaturation reaction is suppressed.
Journal ArticleDOI
Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells
TL;DR: Three heterogeneous RNA species in HeLa cells have been characterized with respect to kinetics of labeling, turnover and location, showing that the nucleoplasmic, cytoplasmic and polyribosome-associated messenger fractions are relatively unaffected by doses of actinomycin which completely inhibit ribosomal RNA synthesis.
Journal ArticleDOI
Structural organization of nuclear complexes containing DNA-like RNA.
TL;DR: It is established that 30 s particles are monomers of a more complex polysome-like structure, which is formed by a long D-RNA strand and a number of globular protein particles bound to it and tightly packed along the D- RNA strand.
Journal ArticleDOI
Rapidly labeled HeLa cell nuclear RNA: I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA
TL;DR: The rapidly labeled RNA from HeLa cells has been further studied in this article, showing that 50% of the [3H]uridine incorporated into RNA in four to five minutes is found in this heterogeneous fraction.