Open Access
Studies on detergent additives of protease enzyme from an estuarine bacterium Bacillus cereus
R. Kanmani,S. Dhivya,S. Jayalakshmi,P. Vijayabaskar +3 more
- Vol. 2, Iss: 7, pp 157-163
TLDR
The present study is an attempt to produce the protease enzyme from the bacterial strain Bacillus cereus, which was isolated from the estuarine sediment sample on casein agar medium and partially purified and lyophilized.Abstract:
The present study is an attempt to produce the protease enzyme from the bacterial strain Bacillus cereus . The strain was isolated from the estuarine sediment sample on casein agar medium. Various cultural conditions were optimized. Based on the results obtained from optimization the mass scale culture was made with pH 9.0; 40 0 C temperature; 35ppt of salinity; 3% of the casein as the suitable substrate. Ammonium sulphate saturation was made and the enzyme was partially purified and lyophilized. 80% saturation has given the maximum saturation of 0.9g. SDS-PAGE was made and there was the separation at 55KDa and 20KDa representing the enzyme protease. Application study was carried out with the enzyme recovered from Bacillus cereus. The purified enzyme was included in to the stain removal procedure. There was the better stain removing observation at the time of using the protease enzyme.read more
Citations
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Microbial Proteases Applications.
TL;DR: The current review focuses on the comparison among different proteases and the current problems faced during production and application at the industrial level to promote microbial proteases economically and commercially around the world.
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Microbial proteases: ubiquitous enzymes with innumerable uses
TL;DR: Proteases have found application in industries besides food, like leather, textiles, detergent, waste management, agriculture, animal husbandry, cosmetics, and pharmaceutics.
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Amylase production by moderately halophilic Bacillus cereus in solid state fermentation
TL;DR: The sugarcane bagasse can be utilized for the cheap production of amylase from Bacillus cereus and is less expensive in comparison to synthetic media and is readily available for production purposes.
Amylase Production Using Bacillus cereus Isolated from a Vermicompost Site
TL;DR: In this paper, the effect of different production parameters such as pH, temperature, carbon source, nitrogen source (Organic and inorganic) incubation time inoculum sizes and surfactants on amylase production by the isolated bacterial strain was studied.
Research Article PRODUCTION AND PARTIAL CHARACTERIZATION OF ALKALINE PROTEASE FROM BACILLUS TEQUILENSIS STRAINS CSGAB0139 ISOLATED FROM SPOILT COTTAGE CHEESE
TL;DR: In this article, an alkaline protease producing strain was isolated from spoilt cottage cheese sample which was identified as Bacillus tequilensis strain SCSGAB0139 on the basis of morphological, cultural, biochemical characteristics and 16S rRNA sequence analysis.
References
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Bergey's Manual of Determinative Bacteriology.
Book
Isolation and characterization
TL;DR: Animal Models and Therapy, Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy, and Use of Differentiating Embryonics Stem cells in the Parkinsonian Mouse Model are reviewed.
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Molecular and Biotechnological Aspects of Microbial Proteases
TL;DR: Despite the extensive research on several aspects of proteases, there is a paucity of knowledge about the roles that govern the diverse specificity of these enzymes and deciphering these secrets would enable to exploit proteases for their applications in biotechnology.
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Bacterial alkaline proteases: molecular approaches and industrial applications
TL;DR: The types and sources of proteases, protease yield-improvement methods, the use of new methods for developing novel proteases and applications of alkaline proteases in industrial sectors are discussed, with an overview on the use in the detergent industry.
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Developments in the use of Bacillus species for industrial production
TL;DR: Developments from the understanding of the complex protein translocation machinery of Gram-positive bacteria should allow the resolution of current secretion challenges and make Bacillus species preeminent hosts for heterologous protein production.
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