Journal ArticleDOI
Studies on lectins. XLIII. Isolation and characterization of the lectin from restharrow roots (Ononis hircina Jacq.).
Reads0
Chats0
TLDR
From 1 kg of dried Ononis hircina Jacq.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1978-03-20. It has received 23 citations till now. The article focuses on the topics: Affinity electrophoresis & Lectin.read more
Citations
More filters
Book ChapterDOI
Lectins in higher plants
Halina Lis,Nathan Sharon +1 more
TL;DR: This chapter discusses physicochemical properties of lectins in higher plants, including their ability to form precipitates with polysaccharides or glycoproteins, either in liquid or semisolid media and their interactions provide information regarding lectin specificity.
Journal ArticleDOI
Qualitative and quantitative applications of affinity electrophoresis for the study of protein—ligand interactions: A review
V. Horˇejsˇi,Marie Tichá +1 more
TL;DR: The method can be used for detection of ligand-binding proteins, checking the purity and binding homogeneity of purified proteins and for determination of dissociation constants of protein—ligand complexes.
References
More filters
Journal ArticleDOI
The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein
TL;DR: In this paper, potato lectin has been purified and shown to be a glycoprotein containing about 50% of carbohydrate, including arabinose, hydroxyproline, half-cystine and phenylalanine.
Journal ArticleDOI
Studies on lectins. XXXV. Water-soluble O-glycosyl polyacrylamide derivatives for specific precipitation of lectins.
TL;DR: The quantitative precipitin curves obtained with these copolymers are similar to those produced by quantitative precipitation of lectins with natural polysaccharides, and may serve as model substances of natural poly Saccharides.
Journal ArticleDOI
Acrylamide gel electrophoresis of soluble plant proteins: a study on pea seedlings in relation to development.
TL;DR: The application of the technique of acrylamide gel electrophoresis to the separation of components of the soluble proteins of plants is described and the reproducibility of the results is demonstrated with reference to Neurospora proteins.
Journal ArticleDOI
Isolation, characterization, and biological activities of five mitogens from pokeweed.
Journal ArticleDOI
Studies on lectins XXXI. Determination of dissociation constants of lectin sugar complexes by means of affinity electrophoresis
TL;DR: The relationship between electrophoretic mobility of lectins and concentration of immobilized and free sugar was used for the calculation of dissociation constants of the various complexes Lectin-immobilized sugar and lectin-free sugar.
Related Papers (5)
Studies of lectins XXXVI. Properties of some lectins prepared by affinity chromatography on O-glycosyl polyacrylamide gels
Vaclav Horejsi,Jan Kocourek +1 more