Journal ArticleDOI
Substrate-dependent autoaggregation of Pseudomonas putida CP1 during the degradation of mono-chlorophenols and phenol.
Alan Farrell,Brid Quilty +1 more
TLDR
Bacterial adherence to hydrocarbons (BATH) assays showed cells grown on the higher concentrations of mono-chlorophenol to be more hydrophobic than those grown on phenol and lower concentrations of Mono- chlorophenol, suggesting that increased hydrophobicity and autoaggregation of P. putida CP1 were a response to toxicity of the added substrates.Abstract:
A bacterium, CP1, identified as Pseudomonas putida strain, was investigated for its ability to grow on and degrade mono-chlorophenols and phenols as sole carbon sources in aerobic shaking batch culture. The organism degraded up to 1.56 mM 2- and 3-chlorophenol, 2.34 mM 4-chlorophenol and 8.5 mM phenol using an ortho-cleavage pathway. P. putida CP1, acclimated to degrade 2-chlorophenol, was capable of 3-chlorocatechol degradation, while P. putida, acclimated to 4-chlorophenol degradation, degraded 4-chlorocatechol. Growth of P. putida CP1 on higher concentrations of the mono-chlorophenols, >or=1.56 mM 4-chlorophenol and >or=0.78 mM 2- and 3-chlorophenol, resulted in decreases in cell biomass despite metabolism of the substrates, and the formation of large aggregates of cells in the culture medium. Increases in cell biomass with no clumping of the cells resulted from growth of P. putida CP1 on phenol or on lower concentrations of mono-chlorophenol. Bacterial adherence to hydrocarbons (BATH) assays showed cells grown on the higher concentrations of mono-chlorophenol to be more hydrophobic than those grown on phenol and lower concentrations of mono-chlorophenol. The results suggested that increased hydrophobicity and autoaggregation of P. putida CP1 were a response to toxicity of the added substrates.read more
Citations
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Journal ArticleDOI
Chlorophenols and other related derivatives of environmental concern: properties, distribution and microbial degradation processes.
TL;DR: This review underscores the mechanisms of chlorophenols biodegradation with the view to understanding how bioremediation processes can be optimized for cleaning up chloroaromatic contaminated environments.
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Integrative approaches for assessing the ecological sustainability of in situ bioremediation
TL;DR: A detailed insight is provided into some of the key factors that affect the efficiency of in situ bioremediation along with a comprehensive account of the integrative approaches used for assessing the ecological sustainability of processes.
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Cell hydrophobicity is a triggering force of biogranulation
TL;DR: The essential role of cell hydrophobicity in the formation of granular sludge is reviewed, and a selection pressure-based inducing strategy for cell hydphobicity was further proposed.
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Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity
TL;DR: Serratia spp.
Journal ArticleDOI
Aerobic granulation for 2,4-dichlorophenol biodegradation in a sequencing batch reactor.
TL;DR: Efficient degradation of 2,4-DCP by the aerobic granules suggests their potential application in the treatment of industrial wastewater containing chlorophenols and other inhibitory chemicals.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Book
Standard methods for the examination of water and wastewater
TL;DR: The most widely read reference in the water industry, Water Industry Reference as discussed by the authors, is a comprehensive reference tool for water analysis methods that covers all aspects of USEPA-approved water analysis.
Book
Manual for the identification of medical bacteria.
S. T. Cowan,K. J. Steel +1 more
TL;DR: Manual for the identification of medical bacteria as discussed by the authors, Manual for identification of Medical bacteria, مرکز فناوری اطلاعات و اشعران رسانی
Journal Article
Manual For The Identification Of Medical Bacteria
TL;DR: A manual for the identification of medical bacteria is presented for the first time in a systematic fashion.
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