Journal ArticleDOI
Sulfidogenesis at Low pH by Acidophilic Bacteria and its Potential for the Selective Recovery of Transition Metals from Mine Waters
TLDR
Biosulfidogenesis (the generation of hydrogen sulfide by microorganisms) in acidic liquors was investigated using two metabolically-distinct bacteria, and the potential of using acidophilic sulfidogens for the selective recovery of metals from acidic waste streams is discussed.Abstract:
Biosulfidogenesis (the generation of hydrogen sulfide by microorganisms) in acidic liquors was investigated using two metabolically-distinct bacteria. One was a novel acidophilic sulfate-reducing bacterium (isolate CL4) that grew at pH 3.0 and above using glycerol as electron donor, and the other was the type strain of Acidithiobacillus ferrooxidans which was grown at pH 2.5 using hydrogen (derived from dissolution of metallic iron) as electron donor and elemental sulfur as electron acceptor. Both bacteria were grown in pH-controlled bioreactors. Isolate CL4 mediated the selective precipitation of zinc in situ, while the At. ferrooxidans bioreactor operated as an off-line system, generating hydrogen sulfide that precipitated copper in a separate reaction vessel. The potential of using acidophilic sulfidogens for the selective recovery of metals from acidic waste streams is discussed.read more
Citations
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Journal ArticleDOI
Sulfate reduction at low pH to remediate acid mine drainage.
Irene Sánchez-Andrea,Irene Sánchez-Andrea,José Luis Sanz,Martijn F.M. Bijmans,Alfons J. M. Stams +4 more
TL;DR: Important factors for biotechnological application of SRB such as the inocula, the pH of the process, the substrates and the reactor design are discussed and microbial communities of sulfidogenic reactors treating AMD which comprise fermentative-, acetogenic- and SRB as well as methanogenic archaea are reviewed.
Journal ArticleDOI
New perspectives in acid mine drainage microbiology
TL;DR: In this paper, it has been shown that acid mine drainage varies significantly from site to site, and spans a wide range of pH, temperature and oxygen content such variations inevitably mean that a variety of acidophilic microorganisms with varying physiological properties can be found in AMD.
Journal ArticleDOI
Desulfosporosinus acidiphilus sp. nov.: a moderately acidophilic sulfate-reducing bacterium isolated from acid mining drainage sediments : New taxa: Firmicutes (Class Clostridia, Order Clostridiales, Family Peptococcaceae).
TL;DR: On the basis of phenotypic, phylogenetic, and genetic characteristics, strain SJ4T represents a novel species within the genus Desulfosporosinus, for which the name Desulfo porosinus acidiphilus sp.
Journal ArticleDOI
Desulfosporosinus acididurans sp. nov.: an acidophilic sulfate-reducing bacterium isolated from acidic sediments
TL;DR: Three strains of sulfate-reducing bacteria (M1T, D, and E) were isolated from acidic sediments and characterized phylogenetically and physiologically and represent a novel species within the genus Desulfosporosinus, for which the name Desulfo porosinus acididurans sp.
Journal ArticleDOI
Precipitation of arsenic sulphide from acidic water in a fixed film bioreactor
Fabienne Battaglia-Brunet,Catherine Crouzet,André Burnol,Stéphanie Coulon,Dominique Morin,Catherine Joulian +5 more
TL;DR: This study was the first to show that As can be removed by bioprecipitation of orpiment from acidic solution containing up to 100mg L(-1) As(V) in a bioreactor.
References
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Journal ArticleDOI
Anaerobic Growth of Thiobacillus ferrooxidans.
TL;DR: The results suggest that ferric iron may be an important electron acceptor for the oxidation of sulfur compounds in acidic environments.
Journal ArticleDOI
Microbial communities and geochemical dynamics in an extremely acidic, metal-rich stream at an abandoned sulfide mine (Huelva, Spain) underpinned by two functional primary production systems
TL;DR: An extremely acidic stream draining an abandoned mine in the Iberian Pyrite Belt, Spain, was ramified with stratified macroscopic gelatinous microbial growths, promoting complex microbial interactions involving transfer of electron donors and acceptors and of organic carbon between microorganisms in the stream water and the gelatinous streamer growths.
Journal ArticleDOI
Anaerobic respiration using Fe(3+), S(0), and H(2) in the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans.
TL;DR: It is shown that the bacterium Acidithiobacillus ferrooxidans could chemolithoautotrophically grow not only on H(2)/O(2) under aerobic conditions but also on H (2)/Fe(3+), H( 2)/S(0), or S(0)/ Fe(3+) under anaerobic conditions.
Journal ArticleDOI
Sulfidogenesis in Low pH (3.8–4.2) Media by a Mixed Population of Acidophilic Bacteria
TL;DR: Analysis of microbial communities, using a combination of total bacterial counts and fluorescent in situ hybridization, confirmed that concurrent growth of both bacteria occurred during sulfidogenesis under strictly anoxic conditions in a pH-controlled fermenter, as well as potential syntrophic interactions, involving hydrogen transfer between M1 and PFBC, are discussed.
Book ChapterDOI
Biological treatment of acid mine drainage
TL;DR: In this article, the authors describe the experience obtained with THIOPAQ technology treating acid mine drainage is described, which involves biological sulfate reduction technology and the removal of heavy metals as metal sulfide precipitates.