Journal ArticleDOI
Synthesis of Cell Wall Microfibrils in vitro by a "Soluble" Chitin Synthetase from Mucor rouxii
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TLDR
A "soluble" form of chitin synthetase was separated from a membrane-rich fraction by exposure to the enzyme substrate (uridine diphosphate N-acetyl-D-glucosamine) and activator (Nacetyl, D-glocosamine), which catalyzed the synthesis of microfibrils similar, if not identical, to those formed by the fungus as discussed by the authors.Abstract:
A "soluble" form of chitin synthetase was separated from a membrane-rich fraction by exposure to the enzyme substrate (uridine diphosphate N-acetyl-D-glucosamine) and activator (N-acetyl- D-glucosamine). The solubilized enzyme catalyzed the synthesis of chitin microfibrils similar, if not identical, to those formed in vivo by the fungus. Cell wall microfibrils were thus abundantly formed in the absence of a living cell or its membranes.read more
Citations
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Chitin: New facets of research
TL;DR: Research on chitin as a marine resource is pointing to novel applications for this cellulose-like biopolymer, and new methods for preparing the bioactive alkyl glycoside of N-acetyl-D-glucosamine and microcrystalline Chitin have encouraged their use as promoters for growth of bifidobacteria and as an aid in digestion of high-lactose cheese whey by domestic animals.
Journal ArticleDOI
Insect chitin: Physical state, synthesis, degradation and metabolic regulation
Book ChapterDOI
Cell-wall synthesis in apical hyphal growth
TL;DR: This chapter discusses the cell wall synthesis in apical hyphal growth and reveals a cytoskeletal organization of the cytoplasm at the apex, which may be crucial to its polarized activity.
Book ChapterDOI
Chitin Metabolism in Insects
TL;DR: This chapter highlights some of the recent and important findings obtained from studies conducted on the synthesis, structure, physical state, modification, organization, and degradation of chitin in insect tissues, as well as the interplay of Chitin with chit in-binding proteins, the regulation of genes responsible for chitIn metabolism, and, finally, the targeting of chItin metabolism for insect-control purposes.
References
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Journal ArticleDOI
Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii.
TL;DR: Comparative studies were made on their composition and structure to explore possible morphogenetic implications and major quantitative differences were found in protein, purine-pyrimidine, and especially mannose content, all of which were higher in the yeast walls.
Journal ArticleDOI
Specificity of Binding of Hexopyranosyl Polysaccharides with Fluorescent Brightener
Hiroshi Maeda,Nakao Ishida +1 more
TL;DR: Infrared spectral change brought about by the binding might provide a clue to the elucidation of the problems concerning the former two alternatives in order to clarify the nature of binding of the brightener on the polysaccharides.
Journal ArticleDOI
ISOLATION OF β-GLUCAN SYNTHETASE PARTICLES FROM PLANT CELLS AND IDENTIFICATION WITH GOLGI MEMBRANES
TL;DR: It is concluded that the synthetase particles from peas that form mainly beta-1,4-glucan from UDPG and GDPG have now been separated from other cell particles by combinations of velocity and isopycnic density gradient centrifugation and are Golgi membranes.
Journal ArticleDOI
Induction of yeastlike development in mucor by carbon dioxide
TL;DR: Vegetative development of Mucor rouxii may follow either one of two patterns of morphogenesis (mold-yeast dimorphism), depending on the atmosphere of incubation, and introduction of CO(2) into an anaerobic atmosphere induced development of spherical, budding yeastlike cells.
Journal ArticleDOI
The synthesis of chitin in cell-free extracts of Neurospora crassa.
Luis Glaser,David H. Brown +1 more
TL;DR: Evidence will be presented that uridine diphosphate-N-acetylglucosamine’ acts as a glycosyl donor in the synthesis of chitin by a cell-free preparation from Neurospora crassa.
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