Showing papers in "Insect Biochemistry in 1986"

TL;DR: The metabolism and distribution of endogenous ecdysone and injected [3H]ecdysone were studied during the pupal-adult development of Manduca sexta and revealed the sequential appearance in the haemolymph and gut of progressively oxidized metabolites of ecDysone, suggestive of both the induction of the steroid hydroxylases (oxidases) by substrate or other effector substances and the possible coordination of developmental events by ecdysteroids other than 20-hyd

TL;DR: Peptidoglycan isolated from Gram-positive bacteria initiated the activation of the enzymes in plasma at a concentration of a few ng/ml, strongly suggest that the active principle of bacterial cell walls is peptidogly can.

TL;DR: Results indicate that prophenoloxidase is synthesized by the oenocytoids.

TL;DR: Data support the proposition that amylase is initially an integral protein in the membrane of cytoplasmic vesicles at the apex of the epithelial cells and is subsequently processed to become soluble and is set free into the midgut lumen by exocytosis.

TL;DR: The cuticular hydrocarbons of Drosophila virilis were isolated and identified by TLC, GC, HPLC and GC-MS and ozonolysis-GC techniques as mentioned in this paper.

TL;DR: The basal lamina over the various regions of the salivary glands is characterized by unique carbohydrates and this suggests that surface receptors may provide the specificity that limits invasion by sporozoites to particular lobes of the Salamander aegypti.

TL;DR: Fat bodies from non-blood-fed females incubated with physiological levels of 20-hydroxyecdysone and the juvenile hormone analog methoprene contained twice as much vitellogenin message as those incubation with 20-HydroxyECDysone alone, while Methoprene alone had no effect.

TL;DR: Methylalkanes were identified in the surface lipids of 3 species of tsetse flies including several that had new sequences of methyl branching, particularly in dimethyltritriacontane of female G. austeni and G. pallidipes.

TL;DR: Photochemical action spectra of Ecdysone 20-monooxygenase activity confirmed the cytochrome P-450 dependency of both the mitochondrial and microsomal ecdys one 20-hydroxylase systems.

TL;DR: The results indicate that the proteins of locust cuticle constitute a group of structural proteins different from other known structural proteins.

TL;DR: Investigation of the fate of injected ecdysteroids or [3H]20-hydroxyecdysone in various species of ticks, spiders, scorpions, myriapods, crustaceans and insects finds some have retention times similar to the apolar conjugates AP2 of the tick, Ornithodoros moubata and could represent inactivation products and/or a hormone storage-form for embryos.

TL;DR: The pulse labeling experiment clearly demonstrated that the metabolic shift from lipogenesis to glycogenesis occurred in fat body at the middle of the last instar; a predominant incorporation was found in lipids when [14C]glucose was injected at the early stage, while at the late stage glycogen synthesis became most active.

TL;DR: Results suggest that the C-2 hydroxylase is a cytochrome P-450 monooxygenase; surprisingly however CO inhibition could not be evidenced even at a CO O 2 ratio of 9:1.

TL;DR: The cellulase from the termite Nasutitermes walkeri consists of two enzymes which predominantly cleaves cellulose randomly to glucose, cellobiose and cellotriose and has some exoglucosidase activity as glucose is the only product produced from cellulose.

TL;DR: Very little label from injected linoleic acid occurred as monounsaturated or saturated fatty acids, indicating only slight, if any, β-oxidation of 18:2 to acetate and subsequent lipid synthesis.

TL;DR: Fat body tissue has been shown to be responsible for the synthesis of the antibacterial proteins in Phormia terranovae.

TL;DR: The data suggest that E. ello larvae and imagoes display a soluble midgut β-fructosidase, which was recognized by its activity toward sucrose and raffinose and lack of activity upon maltose and melibiose.

TL;DR: Ecdysone 20-monooxygenase, the enzyme system that hydroxylates ecdysone to 20-hydroxyecdysone, was characterized in wandering stage larvae of Drosophila melanogaster and blood fed adult female Aedes aegypti using an in vitro radioassay in conjunction with analytical thin layer chromatography.

TL;DR: Results suggested that N-glycosylation of insect proteins, as in mammals, yeasts and plants, involves the en bloc transfer of an oligosaccharide containing the unit (Glu)3(Man)9(GlcNAc)2 from a lipid intermediate to an asparagine residue followed by removal of glucose residues.

TL;DR: Ecdysone 20-monooxygenase, the enzyme system that hydroxylates ecdysone to 20-hydroxyecdysone was characterized in wandering stage larvae of Drosophila melanogaster and blood fed adult female Aedes aegypti using an in vitro radioassay in conjunction with analytical thin layer chromatography as discussed by the authors.

TL;DR: In this article, in vivo and in vitro bioluminescence spectral data are presented for 12 species of Elateridae (two tribes, four genera) in distinct stages of their life cycle.

TL;DR: Using one of the cloned genes as a probe on northern hybridizations, it was found that injection of 20-hydroxyecdysone into non-blood-fed decapitated females stimulated vitellogenin gene expression, and the response was much greater in blood-fed severed females than in non- Blood-fed females.

TL;DR: Results show that diapause eggs have a metabolic complex coupled with carbohydrate and amino acid metabolism inclusive of the 2-oxoglutarate-glutamate shuttle.

TL;DR: In the spermatophore, marked increase of alanine with low concentrations of ornithine and glutamate suggests the presence of a pathway for the active formation of 2-oxoglutarate with pyruvate via glutamate from arginine.

TL;DR: Wild tomato leaves or 2-tridecanone fed to fifth instar larvae of the tobacco budworm, Heliothis virescens, caused a 2.2 to 3.1-fold increase in gut microsomal cytochrome P-450, and glutathione S-transferase activity decreased during the first 24 hr of exposure, then recovered and increased above control levels after 3 days.

TL;DR: Dramatic changes are document in the accumulation and biosynthesis of hydrocarbon and other lipids during larval development of the cabbage looper.

TL;DR: Aspects of hormonal regulation and apoprotein activation of the locust flight muscle lipoprotein lipase are discussed and compared with the model for vertebrate lipop protein lipase.

TL;DR: Cuticular melanization associated with the gregarious phase of the common armyworm larvae, Leucania separata, is caused by a neurohormone, melanization and reddish coloration hormone (MRCH), which shows homology with each other.

TL;DR: Prostaglandins of the E and F series are formed from 20:4 in male and female houseflies during mating, and about 2.1% of the total radioactivity injected into males just prior to mating was transferred to females during mating.