Tamm–Horsfall urinary glycoprotein. The chemical composition
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The glycoprotein was shown to possess a high half-cystine content of 1 per 11-12 amino acid residues, which has been confirmed by performic acid oxidation and S-alkylation with iodoacetate andiodoacetamide.Abstract:
1. A revised amino acid and carbohydrate composition of human Tamm–Horsfall glycoprotein is presented. 2. No significant differences were obtained in the amino acid composition of Tamm–Horsfall glycoprotein isolated from patients with cystic fibrosis. 3. The glycoprotein was shown to possess a high half-cystine content of 1 per 11–12 amino acid residues, which has been confirmed by performic acid oxidation and S-alkylation with iodoacetate and iodoacetamide. No thiol groups were detected in the glycoprotein. 4. Treatment of the glycoprotein with 0.5m-sodium hydroxide at 4°C for 2 days did not release heterosaccharide material, which suggests that the predominant carbohydrate–protein linkages present are not of the O-glycosidic type. 5. No N-terminal amino acid was detected in the glycoprotein.read more
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Journal ArticleDOI
Tamm-Horsfall glycoprotein: biology and clinical relevance
TL;DR: This property supports the notion that abundant THP excretion in urine is promoted in the host by selective pressure to obtain an efficient defense against urinary tract infections caused by uropathogenic bacteria.
Journal ArticleDOI
The Asn-linked carbohydrate chains of human Tamm-Horsfall glycoprotein of one male. Novel sulfated and novel N-acetylgalactosamine-containing N-linked carbohydrate chains.
TL;DR: Human Tamm-Horsfall glycoprotein has been purified from the urine of one male by a combination of three liquid-chromatographic techniques, and the primary structure of 30 N-glycans, including 10 novel oligosaccharides, were determined by 1H-NMR spectroscopy.
Journal ArticleDOI
Tamm-Horsfall Protein Binds to Type 1 Fimbriated Escherichia coli and Prevents E. coli from Binding to Uroplakin Ia and Ib Receptors
TL;DR: It is established, on a functional level, that THP contains conserved high-mannose moieties capable of specific interaction with type 1 fimbriae and strongly suggest that this major urinary glycoprotein is a key urinary anti-adherence factor serving to prevent type 1fimbriated E. coli from binding to the urothelial receptors.
Journal ArticleDOI
Identification of human uromodulin as the Tamm-Horsfall urinary glycoprotein
Diane Pennica,William J. Kohr,Wun Jing Kuang,Debbie Glaister,Bharat B. Aggarwal,Ellson Y. Chen,David V. Goeddel +6 more
TL;DR: Evidence is provided that uromodulin is identical to the previously characterized Tamm-Horsfall glycoprotein, the most abundant protein in normal human urine.
Journal ArticleDOI
Tamm-Horsfall protein--uromodulin (1950-1990).
Satish Kumar,Andrew V. Muchmore +1 more
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