The basement membrane as a structured surface - role in vascular health and disease.
TLDR
The emerging role of the biophysical properties of the basement membrane in regulating critical aspects of vascular health and disease is explored.Abstract:
The basement membrane (BM) is a thin specialized extracellular matrix that functions as a cellular anchorage site, a physical barrier and a signaling hub. While the literature on the biochemical composition and biological activity of the BM is extensive, the central importance of the physical properties of the BM, most notably its mechanical stiffness and topographical features, in regulating cellular function has only recently been recognized. In this Review, we focus on the biophysical attributes of the BM and their influence on cellular behavior. After a brief overview of the biochemical composition, assembly and function of the BM, we describe the mechanical properties and topographical structure of various BMs. We then focus specifically on the vascular BM as a nano- and micro-scale structured surface and review how its architecture can modulate endothelial cell structure and function. Finally, we discuss the pathological ramifications of the biophysical properties of the vascular BM and highlight the potential of mimicking BM topography to improve the design of implantable endovascular devices and advance the burgeoning field of vascular tissue engineering.read more
Citations
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Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology
TL;DR: In this paper, the authors review EC responses to substrate-derived biophysical cues, namely topography, curvature, and stiffness, as well as flow-derived stresses, notably shear stress, pressure, and tensile stresses.
Synthesis and deposition of basement membrane proteins by primary brain capillary endothelial cells in a murine model of the blood-brain barrier
Torben Moos,Lisa Juul Routhe,Svend Birkelund,Annette Burkhart,Allan Stensballe,Maj Schneider Thomsen +5 more
TL;DR: In this paper, the authors analyse four different in vitro models of the murine BBB for expression and possible secretion of major basement membrane proteins from murine BCECs (mBCECs).
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Bioinstructive Layer-by-Layer-Coated Customizable 3D Printed Perfusable Microchannels Embedded in Photocrosslinkable Hydrogels for Vascular Tissue Engineering
Cristiana F. V. Sousa,Catarina A Saraiva,Tiago Ruivo Correia,Tamagno Pesqueira,Sónia G. Patrício,Maria Isabel Rial-Hermida,João Borges,João F. Mano +7 more
TL;DR: In this paper, an alternative approach to bioengineer 3D vascularized constructs by embedding bioinstructive 3D multilayered microchannels, developed by combining 3D printing with the layer-by-layer assembly technology, in photopolymerizable hydrogels.
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Gelatin coating promotes in situ endothelialization of electrospun polycaprolactone vascular grafts
Yuehao Xing,Yongquan Gu,Lianrui Guo,Jianming Guo,Zeqin Xu,Yonghao Xiao,Zhiping Fang,Cong Wang,Zeng-guo Feng,Zhonggao Wang +9 more
TL;DR: Results showed that gelatin coating on the internal surface of PCL grafts is a simple and effective way to promote endothelialization and complete endothelium can inhibit intimal hyperplasia in the long-term.
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Is there a universal mechanism of cell alignment in response to substrate topography
Claire Leclech,Abdul I. Barakat +1 more
TL;DR: In this article, the authors present a model of topography-driven cell alignment which posits that anisotropic topographies impose lateral constraints on the growth of focal adhesions and actin stress fibers.
References
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Flow-mediated endothelial mechanotransduction
TL;DR: The transmission of hemodynamic forces throughout the endothelium and the mechanotransduction mechanisms that lead to biophysical, biochemical, and gene regulatory responses of endothelial cells to hemodynamic shear stresses are reviewed.
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Basement membranes: structure, assembly and role in tumour angiogenesis
TL;DR: The basement membrane (BM) as mentioned in this paper is a specialized form of extracellular matrix (ECM) which mediates tissue compartmentalization and sends signals to epithelial cells about the external microenvironment.
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Basement Membrane Complexes with Biological Activity
Hynda K. Kleinman,Mary L. McGarvey,John R. Hassell,Vicki L. Star,Frances B. Cannon,Gordon W. Laurie,George R. Martin +6 more
TL;DR: Molecular sieve studies on the soluble extract demonstrate that laminin, entactin, and nidogen are associated in large but dissociable complexes which may be a necessary intermediate in the deposition of basement membrane.
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Engineering substrate topography at the micro- and nanoscale to control cell function.
TL;DR: The use of in vitro synthetic cell-nanotopography interactions to control cell behavior and influence complex cellular processes, including stem-cell differentiation and tissue organization are reviewed.
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Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell
TL;DR: The mechanotransduction-induced EC adaptive processes in the straight part of the aorta represent a case of the "Wisdom of the Cell," as a part ofThe more general concept of the 'W Wisdom of the Body' promulgated by Cannon, to maintain cellular homeostasis in the face of external perturbations.
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