Journal ArticleDOI
The Complexity of Microbial Metal Nanoparticle Synthesis: A Study of Candida parapsilosis ATCC 7330 mediated Gold Nanoparticles Formation
TLDR
Characterization of the bio-synthesized gold nanoparticles using X-ray photoelectron spectroscopy and elemental analysis revealed that nanoparticles are stabilized by proteins, implying that free –SH increase aggregation and emphasize this as a possible strategy to produce monodisperse gold nanoparticle in biological extracts which is otherwise difficult.Abstract:
Understanding the biosynthetic mechanism of gold nanoparticle formation is the key to controlling the size, dispersity, and morphology of the nanoparticles. Reduction of gold (III) to gold (0) in cell-free extracts of Candida parapsilosis ATCC 7330 is not only enzymatic, as confirmed by experiments with heat denatured extracts. In addition to proteins, cellular reducing equivalents also contribute to the formation of gold nanoparticles in a concentration-dependent manner. Characterization of the bio-synthesized gold nanoparticles using X-ray photoelectron spectroscopy and elemental analysis revealed that nanoparticles are stabilized by proteins. The importance of protein three-dimensional structure in producing stable gold nanoparticles is also addressed. Making free thiol groups (–SH) unavailable by derivatizing them in protein extracts resulted in monodisperse gold nanoparticles implying that free –SH increase aggregation and emphasize this as a possible strategy to produce monodisperse gold nanoparticles in biological extracts which is otherwise difficult.read more
Citations
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Journal ArticleDOI
Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage
TL;DR: In this article , a review of studies on the antimicrobial and biosynthesis mechanisms of metal nanoparticles were compiled and evidence-based mechanisms proposed, revealing the importance of enzymatic disturbance by internalized metal ions, while the role of reducing and negatively charged molecules.
Book ChapterDOI
Bacterial cellular mechanisms for synthesis of green nanostructured compounds
TL;DR: In this paper , the authors mainly focus on the mechanism of biosynthesis of nanoparticles from the bacterial system and its broad applications, including the microbial route of NPs, including bacteria, fungi, cyanobacteria, and others with their uses.
References
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Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent
Jozef Sedlak,Raymond H. Lindsay +1 more
TL;DR: A simple spectrophotometric method for the routine concomitant determination of sulfhydryl groups in PB- SH, NP-SH, and T-SH fractions in various tissues is reported.
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Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine
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Quantitative Chemical Analysis
TL;DR: The results of typical analyses obtained in the laboratories of the authors occupy eleven pages, and will prove, useful to analysts and others for reference and guidance; the list of important works of reference provided will also be equally serviceable.
Journal ArticleDOI
Preparation and Characterization of Au Colloid Monolayers
TL;DR: In this article, the design and initial characterization of two-dimensional arrays of colloidal Au particles are reported, which are prepared by self-assembly of 12 nm diameter colloidal particles onto immobilized polymers having pendant functional groups with high affinity for Au (i.e., CN, SH, and NH 2 ).
Journal ArticleDOI
Antimicrobial activity of metals: mechanisms, molecular targets and applications
TL;DR: The chemical and toxicological principles that underlie the antimicrobial activity of metals are described and the preferences of metal atoms for specific microbial targets are discussed.