Journal ArticleDOI
The effect of bone morphogenetic protein-2, bone morphogenetic protein-7, parathyroid hormone, and platelet-derived growth factor on the proliferation and osteogenic differentiation of mesenchymal stem cells derived from osteoporotic bone.
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BMP-7 and PDGF-BB (in high doses) could be considered most potentially advantageous because they enhance both proliferation and osteogenic differentiation of MSCs derived from elderly osteoporotic bone.Abstract:
Introduction: It has been previously shown that in patients with osteoporosis, mesenchymal stem cell (MSC) growth rate and osteogenic potential is decreased contributing to inferior fracture consolidation. The aim of this study was to investigate the effect of bone morphogenetic protein-2 (BMP-2), BMP-7, parathyroid hormone (PTH), and platelet-derived growth factor (PDGF) on proliferation and osteogenic differentiation of MSCs derived from patients with osteoporosis. Materials and Methods: Trabecular bone was obtained from 10 patients (four males, mean age 76 years) with lower extremity osteoporotic fractures. MSCs were isolated by enzymatic digestion. Functional assays of proliferation and osteogenic differentiation were performed under the influence of a wide range of concentrations of BMP-2, BMP-7, PTH, and PDGF-BB. Proliferation was assessed using CFU-F and XTT assays. Osteogenic differentiation was assessed by alkaline phosphatase activity and total calcium production. Results: MSC proliferation was found to be stimulated by supplementation with BMP-7 and PDGF-BB, whereas BMP-2 and PTH had little effect. The largest increase in proliferation rate was observed after administration 100 ng/mL of BMP-7. All four molecules induced alkaline phosphatase activity and calcium production in growing osteoblasts with a dose-dependent effect noted. BMP-2 and BMP-7 at their highest studied concentration (100 ng/mL) produced a threefold increase in the osteogenic potential of MSCs. Conclusion: BMP-7, BMP-2, PTH, and PDGF-BB were observed to have a positive effect on osteogenic differentiation of MSCs. BMP-7 and PDGF-BB (in high doses) could be considered most potentially advantageous because they enhance both proliferation and osteogenic differentiation of MSCs derived from elderly osteoporotic bone.read more
Citations
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Journal ArticleDOI
The Transcriptional Profile of Mesenchymal Stem Cell Populations in Primary Osteoporosis Is Distinct and Shows Overexpression of Osteogenic Inhibitors
Peggy Benisch,Tatjana Schilling,Ludger Klein-Hitpass,Sönke P. Frey,Lothar Seefried,N. Raaijmakers,Melanie Krug,Martina Regensburger,S. Zeck,Thorsten Schinke,Michael Amling,Regina Ebert,Franz Jakob +12 more
TL;DR: The results suggest that in primary osteoporosis the transcriptomes of hMSC populations show distinct signatures and little overlap with non-osteoporotic aging, although some hints for senescence-associated changes are detected.
Journal ArticleDOI
Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair.
Cionne N. Manning,Andrea G. Schwartz,Wenying Liu,Jingwei Xie,Necat Havlioglu,Shelly E. Sakiyama-Elbert,Matthew J. Silva,Younan Xia,Richard H. Gelberman,Stavros Thomopoulos +9 more
TL;DR: The novel layered scaffold developed has the potential for improving tendon healing due to its ability to deliver both cells and growth factors simultaneously in a surgically convenient manner.
Journal ArticleDOI
Parathyroid hormone induces differentiation of mesenchymal stromal/stem cells by enhancing bone morphogenetic protein signaling.
TL;DR: It is demonstrated that PTH enhancement of MSC differentiation to the osteoblast lineage occurs through a PTH‐ and LRP6‐dependent pathway by endocytosis of the PTH1R/LRp6 complex, allowing enhancement of BMP signaling.
Journal ArticleDOI
Local drug delivery for enhancing fracture healing in osteoporotic bone.
TL;DR: The aim of this paper is to present the state of the art on local drug delivery in osteoporotic fractures and identify promising new candidate molecules and innovative approaches.
Journal ArticleDOI
Osteogenesis induced by a bone forming peptide from the prodomain region of BMP-7.
Hyung Keun Kim,Ji Hyun Kim,Dae Sung Park,Kyung-Soon Park,Seong Soo Kang,Jun Sik Lee,Myung Ho Jeong,Taek Rim Yoon +7 more
TL;DR: A new peptide sequence is found, called bone forming peptide-1 (BFP-1) and have more high activities of osteogenic differentiation compared with BMP-7, which indicates a new insight into peptides from the immature region of B MP-7 can also be useful in the development of adjuvant therapies for bone-related diseases.
References
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Teriparatide for Acceleration of Fracture Repair in Humans: A Prospective, Randomized, Double-Blind Study of 102 Postmenopausal Women With Distal Radial Fractures
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Journal ArticleDOI
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Journal ArticleDOI
Increased bone formation by intermittent parathyroid hormone administration is due to the stimulation of proliferation and differentiation of osteoprogenitor cells in bone marrow
Saburo Nishida,Akira Yamaguchi,Tatsuhiko Tanizawa,Naoto Endo,T. Mashiba,Y. Uchiyama,Tatsuo Suda,Shusaku Yoshiki,Hideaki E. Takahashi +8 more
TL;DR: The mechanism of the anabolic effect of parathyroid hormone (PTH) on bone formation, human PTH(1-34) was injected subcutaneously to 9-week-old rats 5 times a week for 1 or 3 weeks, and the parameters related to bone formation and bone resorption were significantly increased.