The effect of ionomycin on calcium fluxes in sarcoplasmic reticulum vesicles and liposomes.
TLDR
Ionomycin, a recently discovered calcium ionophore, inhibits the ATP-dependent active Ca2+ transport of rabbit sarcoplasmic reticulum vesicles at concentrations as low as 10(-8) to 10(-6) M.About:
This article is published in Journal of Biological Chemistry.The article was published on 1979-07-25 and is currently open access. It has received 62 citations till now. The article focuses on the topics: Calcium ATPase & Calcium flux.read more
Citations
More filters
Journal ArticleDOI
Relationship of thyrotropin-releasing hormone-induced spike and plateau phases in cytosolic free Ca2+ concentrations to hormone secretion. Selective blockade using ionomycin and nifedipine.
Paul R. Albert,A H Tashjian +1 more
TL;DR: It is demonstrated that the TRH-induced spike in [Ca2+]i in GH cells is caused by release of an ionomycin-sensitive pool of cellular Ca2+ with a small component due to influx of extracellular Ca2+.
Journal ArticleDOI
Ca2+-dependence of conoid extrusion in Toxoplasma gondii tachyzoites
TL;DR: The results suggest that Ca2+‐release from internal stores may act as a key signal to activate a mechanism of conoid extrusion probably mediated, at least in part, by actin‐filaments.
Journal ArticleDOI
Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria.
TL;DR: In conclusion, ionomycin was shown to be an effective ionophore for Ca2+ in rat liver mitochondria and mediated an efflux of K+ provided that the mitochondria were depleted of their endogenous divalent metal ions.
Journal ArticleDOI
Inactivation of anoctamin-6/Tmem16f, a regulator of phosphatidylserine scrambling in osteoblasts, leads to decreased mineral deposition in skeletal tissues
Harald W. A. Ehlen,Milana Chinenkova,Markus Moser,Hans-Markus Munter,Yvonne Krause,Stefanie Gross,Bent Brachvogel,Manuela Wuelling,Uwe Kornak,Andrea Vortkamp +9 more
TL;DR: This study is the first to the authors' knowledge to reveal the requirement of Ano6 in PS scrambling in osteoblasts, supporting a function of PS exposure in the deposition of hydroxyapatite.
Journal ArticleDOI
Selective stimulation of the synthesis of an 80,000-dalton protein by calcium ionophores.
TL;DR: It is suggested that ionomycin selectively increases the cellular concentration of mRNA, which codes for the 80,000-dalton protein, which is probably mediated through an increase in cytoplasmic [Ca2+] caused by the ionophore.
References
More filters
Journal ArticleDOI
Characterization of ionomycin as a calcium ionophore.
C Liu,T E Hermann +1 more
TL;DR: Measurement of the binding of Ionomycin with Ca2+ indicates that ionomycin complexes and transports calcium ion in a one to one stoichiometry.
Journal ArticleDOI
Sarcoplasmic reticulum. IX. The permeability of sarcoplasmic reticulum membranes.
P. F. Duggan,A. Martonosi +1 more
TL;DR: Inulin penetration occurred at pH 7.0 with 1 mM EDTA and at pH 9.0 without EDTA, suggesting increased permeability of FSR membranes, which accords with the higher rate of Ca++ release from FSR at temperatures over 30°C, and the penetration of microsomal membranes by anions is markedly influenced by charge effects.
Journal ArticleDOI
Purification of arsenazo III, a Ca2+-sensitive dye.
TL;DR: Methods are described for detection of the colored impurities using DEAE-cellulose thinlayer chromatography in semiorganic solvents, along with methods for purification of the dye using DEae- cellulose column chromatography and Chelex-100 ion-exchange resin.
Related Papers (5)
Synthesis of ATP coupled to Ca2+ release from sarcoplasmic reticulum vesicles
Rivka Panet,Zvi Selinger +1 more