Journal ArticleDOI
The effects of heparin on endogenous DNA polymerase activity of rat liver nuclei and chromatin fractions.
Robert T. Cook,M. Aikawa +1 more
TLDR
The kinetic data combined with the behavior of the high specific activity chromatin fraction indicate that the heparin effect on endogenous DNA synthesis may have more than one component.About:
This article is published in Experimental Cell Research.The article was published on 1973-04-01. It has received 53 citations till now. The article focuses on the topics: Chromatin & DNA polymerase.read more
Citations
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Journal ArticleDOI
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
Journal ArticleDOI
Heparin binding sites in the human spermatozoa membrane.
TL;DR: The existence in the human spermatozoa membrane of receptorlike functional group for heparin was studied and autoradiography experiments showed that labeling was almost completely restricted to sperm cells in the process of nuclear decondensation.
Journal ArticleDOI
Species Specific Decondensation of Human Spermatozoa Nuclei by Heparin
TL;DR: Electron microscope observations of human spermatozoa nuclei treated with heparin revealed that the chromatin is organized into "hublike" nuclear bodies joined by a network of cross-linked and branched chromatin fibers ranging in thickness from 25 to 1.5 nm.
Journal ArticleDOI
Influences of anionic polysaccharides on DNA synthesis in isolated nuclei and by DNA polymerase α: Correlation of observed effects with properties of the polysaccharides
TL;DR: Heparin and chondroitin sulfate H, which at low concentrations stimulate DNA synthesis in liver nuclei, inhibited DNA synthesis by calf thymus DNA polymerase alpha at all concentrations.
Journal ArticleDOI
The regulation of nuclear DNA template restrictions by acidic polymers.
TL;DR: The models which are proposed in the studies may provide new insight into the regulation of DNA synthesis in mammalian cells and the role of nuclear acidic proteins as one of the important factors in regulating nucleic acid synthesis in the cell.
References
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Journal ArticleDOI
Phosphorus compounds in animal tissues; extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid.
Journal ArticleDOI
Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
Günter Blobel,Van R. Potter +1 more
TL;DR: A procedure is described that gives almost quantitative separation of clean nuclei from a homogenate of rat liver and permits further quantitative fractionation of cytoplasmic components.
Journal ArticleDOI
Studies of Nuclear Acidic Proteins EVIDENCE FOR THEIR PHOSPHORYLATION, TISSUE SPECIFICITY, SELECTIVE BINDING TO DEOXYRIBONUCLEIC ACID, AND STIMULATORY EFFECTS ON TRANSCRIPTION
TL;DR: Methods are described for the extraction, separation, and electrophoretic analysis of a class of acidic nuclear proteins from various tissues of the rat and relationships are observed between DNA binding and enhancement of RNA synthesis.
Journal ArticleDOI
Selective dissociation of histones from calf thymus nucleoprotein
TL;DR: The results suggest that the histones are somewhat heterogeneously distributed along the DNA chain, and imply that both electrostatic and non-electrostatic interactions contribute to the strength of the binding between histones and DNA.
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