Journal ArticleDOI
The isolation of mutants of Aspergillus flavus and A.parasiticus with altered aflatoxin producing ability.
Joan W. Bennett,L. A. Goldblatt +1 more
TLDR
Two methods of isolating mutants of Aspergillus in which aflatoxin production is attenuated or lost are presented and mutants are selected on the basis of altered fluorescence under ultraviolet light.Abstract:
Mutations affecting the production of aflatoxin might be useful in studying its biosynthesis, and in understanding the wide variation in aflatoxigenicity of fungal strains isolated from nature. Two methods of isolating mutants of Aspergillus in which aflatoxin production is attenuated or lost are presented. In one method mutants are selected on the basis of altered fluorescence under ultraviolet light. The second method utilizes a mutant strain which produces aflatoxin and an orange-red mycelial pigment not detected in the wild type. Unpigmented mutants derived from this strain exhibit lowered or lost aflatoxigenicity.read more
Citations
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Journal ArticleDOI
Current Understanding on Aflatoxin Biosynthesis and Future Perspective in Reducing Aflatoxin Contamination
TL;DR: Most, if not all, of the aflatoxin pathway genes are cloned, indicating that the current status of research progress and future possibilities that may be used for solving a Flatoxin contamination are summarized.
Journal ArticleDOI
Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis.
TL;DR: DNA isolated from the wild-type aflatoxin-producing fungus Aspergillus parasiticus NRRL 5862 was used to construct a cosmid genomic DNA library employing the homologous gene (pyrG) encoding orotidine monophosphate decarboxylase for selection of fungal transformants, revealing striking similarity with Streptomyces ketoreductases involved in polyketide biosynthesis.
Journal ArticleDOI
Aflatoxins in stored maize and rice grains in Liaoning Province, China
TL;DR: The aflatoxin content in maize and rice is much lower than the regulated maximum amount allowed in foodstuffs in China and other countries, and these grains are safe for human and livestock consumption and for trading.
Journal ArticleDOI
Physical and transcriptional map of an aflatoxin gene cluster in Aspergillus parasiticus and functional disruption of a gene involved early in the aflatoxin pathway.
TL;DR: The predicted amino acid sequence of two regions of gene-1 showed a high degree of identity and similarity with the beta-ketoacyl-synthase and acyltransferase functional domains of polyketide synthases, consistent with a proposed role for gene- 1 in polyketid backbone synthesis.
Book ChapterDOI
New Perspectives on Aflatoxin Biosynthesis
TL;DR: The preliminary data on toxicity and mutagenicity indicate that the evolution of the aflatoxin pathway may be a response to selective pressures from mycophagous species in nature, and this system may be uniquely suited among micrcbial secondary pathways for testing this ecological hypothesis.
References
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Journal ArticleDOI
Aflatoxin formation by Aspergillus flavus.
Book ChapterDOI
Intermediary metabolism and antibiotic synthesis.
TL;DR: This chapter discusses intermediary metabolism and antibiotic synthesis, and defines secondary metabolites as having, by contrast, a restricted distribution and no obvious function in general metabolism.
Journal ArticleDOI
Production of aflatoxins in submerged culture.
R. I. Mateles,J. C. Adye +1 more
TL;DR: Concentrations of aflatoxin of 60 to 80 mg per liter can readily be obtained in indented shake flasks; somewhat lower yields were obtained in 5-liter fermentors.
Journal ArticleDOI
Aflatoxin production by Aspergillus flavus as related to various temperatures.
A. F. Schindler,A. F. Schindler,John G. Palmer,John G. Palmer,W. V. Eisenberg,W. V. Eisenberg +5 more
TL;DR: Two aflatoxin-producing isolates of Aspergillus flavus were grown for 5 days on Wort media at 2, 7, 13, 18, 24, 29, 35, 41, 46, and 52 C and color of CHCl(3) extracts appeared to be directly correlated with aflatoxins concentrations.