The performance of human mesenchymal stem cells encapsulated in cell-degradable polymer-peptide hydrogels
Sarah B. Anderson,Chien-Chi Lin,Chien-Chi Lin,Donna V. Kuntzler,Kristi S. Anseth,Kristi S. Anseth +5 more
TLDR
The ability to culture and differentiate hMSCs in MMP-degradable hydrogels polymerized via a thiol-ene reaction scheme is demonstrated and that increased cell-mediated hydrogel degradability facilitates directed differentiation of h MSCs.About:
This article is published in Biomaterials.The article was published on 2011-05-01 and is currently open access. It has received 338 citations till now. The article focuses on the topics: Self-healing hydrogels.read more
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Thiol–ene “click” reactions and recent applications in polymer and materials synthesis: a first update
TL;DR: A recent review as discussed by the authors highlights recent applications of thiol-ene "click" chemistry as an efficient tool for both polymer/materials synthesis as well as modification and highlights many of the new and exciting applications where researchers have applied thiolene chemistry in advanced macromolecular engineering and materials chemistry.
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Click hydrogels, microgels and nanogels: Emerging platforms for drug delivery and tissue engineering
TL;DR: Recent exciting developments in click hydrogels, microgels and nanogels, as well as their biomedical applications such as controlled protein and drug release, tissue engineering, and regenerative medicine are presented and discussed.
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Designing degradable hydrogels for orthogonal control of cell microenvironments
TL;DR: This review provides insight into emerging degradable and cell-compatible hydrogels for understanding and modulating cell behavior for various bioengineering applications.
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Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Guoyou Huang,Fei Li,Xin Zhao,Yufei Ma,Yuhui Li,Min Lin,Guorui Jin,Tian Jian Lu,Guy M. Genin,Feng Xu +9 more
TL;DR: This review encapsulates where recent advances appear to leave the ever-shifting state of the art in the cell microenvironment, and it highlights areas in which substantial potential and uncertainty remain.
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Adaptable Hydrogel Networks with Reversible Linkages for Tissue Engineering
Huiyuan Wang,Sarah C. Heilshorn +1 more
TL;DR: An overview of adaptable‐hydrogel design considerations and linkage selections is presented, with a focus on various cell‐compatible crosslinking mechanisms that can be exploited to form adaptable hydrogels for tissue engineering.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
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Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue
TL;DR: A modified form of the dim methylmethylene blue assay is described that has improved specificity for sulphated glycosaminoglycans, and it is shown that in conjunction with specific polysaccharidases, the dimethylmethyleneblue assay can be used to quantitate individual sulphated sugarcans.
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Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture
TL;DR: The use of both synthetic and natural hydrogels as scaffolds for three-dimensional cell culture as well as synthetic hydrogel hybrids that incorporate sophisticated biochemical and mechanical cues as mimics of the native extracellular matrix are discussed.