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Journal ArticleDOI

The Precipitation of Proteins by Synthetic Detergents1a

Frank W. Putnam, +1 more
- 01 May 1944 - 
- Vol. 66, Iss: 5, pp 692-697
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This article is published in Journal of the American Chemical Society.The article was published on 1944-05-01. It has received 126 citations till now. The article focuses on the topics: Precipitation.

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Reversible Denaturation of Enzymes by Sodium Dodecyl Sulfate

TL;DR: Successful renaturation of several oligomeric enzymes after denaturation by sodium dodecyl sulfate is described and the results obtained suggest a general procedure for reactivation of enzymes after treatment with this detergent.
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Nuclease detection in SDS-polyacrylamide gel electrophoresis.

TL;DR: Nucleases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels containing DNA or RNA, by incubating the gels in buffer to remove SDS and to allow renaturation and enzymatic activity.
Journal ArticleDOI

Polymer/surfactant interactions

TL;DR: In this paper, an analysis of the solubility diagrams of a cationic hydroxyethyl cellulose derivative, and a homologous series of sodium alkyl sulfates, has provided a value of the adsorption energy, Φ, of these surfactants into the first layer.
Journal ArticleDOI

Cationic polymer/anionic surfactant interactions☆

TL;DR: In this article, the interaction of a water-soluble cationic substituted cellulose ether with SDS has been examined through a study of precipitation patterns and measurements of surface tension, foaming, and electrophoretic mobility.
Journal ArticleDOI

Ionic Surfactant Aggregates in Saline Solutions : Sodium Dodecyl Sulfate (SDS) in the Presence of Excess Sodium Chloride (NaCl) or Calcium Chloride (CaCl2)

TL;DR: The presence of CaCl(2) induces more compact and densely packed micelles with a significant reduction in gauche defects in the SDS hydrocarbon chains in comparison with NaCl and significantly more stable salt bridges between the charged SDS head groups mediated by Ca(2+) than Na(+).
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