Journal ArticleDOI
The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus.
Mark E. Frankel,Walter Gerhard +1 more
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Heterogeneity constants for the reaction pairs are presented and are consistent with the interaction of monoclonal antibodies with homogeneous protein determinants.About:
This article is published in Molecular Immunology.The article was published on 1979-02-01. It has received 190 citations till now. The article focuses on the topics: Binding constant & Monoclonal antibody.read more
Citations
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Journal ArticleDOI
Grafting of “Abbreviated” Complementarity-Determining Regions Containing Specificity-Determining Residues Essential for Ligand Contact to Engineer a Less Immunogenic Humanized Monoclonal Antibody
Roberto De Pascalis,Makoto Iwahashi,Midori Tamura,Eduardo A. Padlan,Noreen R. Gonzales,Ameurfina D. Santos,Mariateresa Giuliano,Peter Schuck,Jeffrey Schlom,Syed V. S. Kashmiri +9 more
TL;DR: The final variant of the HuCOL-1, which retains its Ag-binding reactivity and shows significantly lower serum reactivity than that of the parental Ab, can serve as a prototype for the development of a potentially useful clinical reagent.
Journal ArticleDOI
Monoclonal antibodies for analysis of the HLA system.
TL;DR: III Antibodies A Monoclonal antibodies against determinants of the HLA-A,B,C glycoproteins and pjmicroglobulin and pJmicroglOBulin against species specific, non-polymorphic determinants 2.
Citation-classic - monoclonal-antibodies for analysis of the hla system
TL;DR: In this paper, a monoclonal antibody against determinants of the HLA-A,B,C glycoproteins and pjmicroglobulin was developed.
Journal ArticleDOI
Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies
TL;DR: Analysis of 34 mutant viruses selected has enabled us to define four antigenic sites on the haemagglutinin molecule that have undergone antigenic drift to a different extent in nature, which implies that the mechanisms responsible for antigenic Drift act selectively on distinct structures of the HA molecule.
Journal Article
Construction, Binding Properties, Metabolism, and Tumor Targeting of a Single-Chain Fv Derived from the Pancarcinoma Monoclonal Antibody CC49
Diane E. Milenic,Takashi Yokota,David Filpula,M. A. J. Finkelman,S. W. Dodd,James F. Wood,Marc Whitlow,Philip Snoy,Jeffrey Schlom +8 more
TL;DR: The construction and characterization of a recombinant single-chain Fv (sFv) of CC49 was shown to be a Mr 27,000 homogeneous entity which could be efficiently radiolabeled with 125I or 131I and shown to bind biopsies of TAG-72-expressing tumors and may have utility in diagnostic and perhaps therapeutic applications for a range of human carcinomas.
References
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Journal ArticleDOI
The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Journal ArticleDOI
Disquisitions on original antigenic sin : i. evidence in man
TL;DR: The basic difference between primary and secondary reactivity rests on the presence of a trapping mechanism that allows anamnestic production of antibody against lower doses of the homologous antigen, thus preventing a standard primary response and allowing manifestations of Original Antigenic Sin.
Book ChapterDOI
Immunologic Specificity and Molecular Structure1
TL;DR: This chapter formulates the basic concepts for understanding immunologic specificity and stresses the probable importance of apolar interactions in the antigen-antibody reactions.
Journal ArticleDOI
Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 [HON1] influenza virus with monoclonal antibodies
Walter Gerhard,Robert G. Webster +1 more
TL;DR: The results are compatible with the idea that the HA of PR8 has available a large repertoire of antigenic modifications that may result from single amino acid substitutions, and that antigenic changes can occur in the strain- specific determinants on the HA molecule in the absence of concomitant changes in the cross-reactive HA determinants.
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Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more