Journal ArticleDOI
The response of BHK21 cells to infection with type 12 adenovirus: II. Relationship of virus-stimulated DNA synthesis to other viral functions☆
TLDR
The results are taken to indicate that the virus initiates chromosomal DNA synthesis but that infection eventually results in extensive destruction of the cell genome, and thus the death of the cells.About:
This article is published in Virology.The article was published on 1969-12-01. It has received 85 citations till now. The article focuses on the topics: DNA synthesis & Mitosis.read more
Citations
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Human cytomegalovirus infection inhibits cell cycle progression at multiple points, including the transition from G1 to S.
M Lu,Thomas Shenk +1 more
TL;DR: Analysis of the cellular DNA content of infected cells by flow cytometry demonstrated that cytomegalovirus does not arrest cell cycle progression at a single point, suggesting that at least two blockages occur in the G1 phase of the cell cycle.
Journal ArticleDOI
Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA.
K Van Doren,D Hanahan,Y Gluzman +2 more
TL;DR: Recombinant viral genomes carrying a selectable drug resistance marker have been constructed by insertion of a hybrid gene for neomycin resistance into the helper-independent adenovirus vector, delta E1/X, demonstrating that early region 1 is not essential for integration of the viral genome.
Journal ArticleDOI
Cell cycle control of the human HSP70 gene: implications for the role of a cellular E1A-like function.
TL;DR: It is suggested that the E1A-like cellular activity may govern certain aspects of cell cycle transcription, particularly at the level of transcription as measured in nuclear runoff assays.
Journal ArticleDOI
Adenovirus-induced alterations of the cell growth cycle: a requirement for expression of E1A but not of E1B.
Antony W. Braithwaite,B. F. Cheetham,P Li,C R Parish,L. K. Waldron-Stevens,Alan J.D. Bellett +5 more
TL;DR: The results suggest that alteration of cell cycle progression is a direct effect of E 1A unrelated to its control of other viral early regions, and may be the function of E1A in transformation.
Journal ArticleDOI
Infection of mouse liver by human adenovirus type 5.
Sylvia Jean Duncan,F. C. A. Gordon,D. W. Gregory,J. L. McPhie,R. Postlethwaite,Ruth White,H. N. A. Willcox +6 more
TL;DR: It is concluded that human adenovirus type 5 undergoes an abortive but lytic infection in most liver cells but that replication may proceed to completion in a few.
References
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Journal ArticleDOI
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
TL;DR: A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
Journal ArticleDOI
A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
Journal ArticleDOI
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane
David Gillespie,Sol Spiegelman +1 more
TL;DR: An improved method for the formation of DNA—RNA hybrids is described, quantitatively more certain than annealing in liquid, since the competing DNA renaturation reaction is suppressed.
Related Papers (5)
Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12.
Induction of DNA synthesis by adenoviruses in contact-inhibited hamster cells
Hiroto Shimojo,Tadashi Yamashita +1 more