The two subunits of penicillin acylase are processed from a common precursor
TLDR
In this paper, les deux sous-unites de la penicilline acylase d'Escherichia coli proviennent d'un precurseur commun polypeptidique localise exclusivement dans la membrane.Abstract:
Les resultats montrent que les deux sous-unites de la penicilline acylase d'Escherichia coli proviennent d'un precurseur commun polypeptidique localise exclusivement dans la membraneread more
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Molecular Regulation of β-Lactam Biosynthesis in Filamentous Fungi
TL;DR: A comparison with the regulatory mechanisms (regulatory proteins and DNA elements) involved in the regulation of genes of primary metabolism in lower eukaryotes is thus of great interest and can be expected to have a major impact on rational strain improvement programs.
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Cloning and characterization of the acyl-coenzyme A: 6-aminopenicillanic-aid-acyltransferase gene of Penicillium chrysogenum
J. L. Barredo,P. van Solingen,Bruno Díez,Emilio Alvarez,Jesús M. Cantoral,A. Kattevilder,E.B. Smaal,Martinus Antonius Math Groenen,Annemarie Eveline Veenstra,Juan F. Martín +9 more
TL;DR: The aat gene of P. chrysogenum resembles the genes encoding Pn acylases of Escherichia coli, Proteus rettgeri and Pseudomonas sp.
Journal ArticleDOI
Cloning and characterization of the genes for two distinct cephalosporin acylases from a Pseudomonas strain.
TL;DR: A DNA library of this strain was constructed in Escherichia coli and screened for the ability to deacylate GL-7ACA to 7ACA, and the acyII-encoded enzyme was found to yield 7ACA from cephalosporin C by direct deacylation.
Journal ArticleDOI
Polycistronic Viral Vectors
TL;DR: The description and comparison of the strategies for co-expression of two genes in bicistronic vectors, from the oldest to the more recently described, and a classification of these strategies based upon either the use of multiple transcripts (with transcriptional mechanisms), or single transcripts (using translational/post-translational mechanisms).
Journal ArticleDOI
The role of penicillin amidases in nature and in industry
TL;DR: Clues to the biological role of this enzyme have been provided, as well as new strategies for the commercial production and utilization of PA, revealing a variety of interesting features that are unique among microorganisms.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Journal ArticleDOI
The penicillin acylase from Escherichia coli ATCC11105 consists of two dissimilar subunits
TL;DR: L'enzyme penicilline acylase d'E.