Journal ArticleDOI
The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of man.
TLDR
Chromosome preparations treated for short periods with the proteolytic enzyme trypsin show well defined banding patterns, comparable to those obtained by more elaborate techniques, and it is possible to map in detail the position of chromosome rearrangements.Abstract:
Chromosome preparations treated for short periods with the proteolytic enzyme trypsin show well defined banding patterns, comparable to those obtained by more elaborate techniques.—With such patterns it is possible to map in detail the position of chromosome rearrangements.—A rare balanced A1–E18 translocation in a phenotypically normal female and the unbalanced product in her abnormal child has been used to demonstrate this mapping method.read more
Citations
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Journal ArticleDOI
Isolation of a human prostate carcinoma cell line (DU 145).
TL;DR: Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.
Journal ArticleDOI
Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas
TL;DR: The karyotypes of cells from 10 Burkitt lymphoma biopsies, eight cell lines established from BL and nine cell lines from non‐BL sources were studied by chromosome banding techniques and it is suggested that the chromosome 14 marker represents a translocation between chromosomes 8 and 14,t (8q‐; 14q+).
Journal ArticleDOI
Chromosome banding in Amphibia
TL;DR: A cytogenetic study performed on a population of the South American leptodactylid frog Eleutherodactylus maussi revealed multiple sex chromosomes of the X1X1X2X2♀/X1 X2Y♂ (=XXAA/XXAY♂) type, which is interpreted as the ancestral condition, preceding the occurrence of the Y-autosome fusion.
Journal ArticleDOI
Identification and Clonal Characterisation of a Progenitor Cell Sub-Population in Normal Human Articular Cartilage
Rebecca Williams,Ilyas M. Khan,Kirsty Richardson,Larissa Nelson,Helen Elizabeth McCarthy,Talal Analbelsi,Sim K. Singhrao,Gary P. Dowthwaite,Rhiannon E. Jones,Duncan M. Baird,Holly Lewis,S. H. Roberts,Hannah Shaw,Jayesh Dudhia,John A. Fairclough,Timothy W. R. Briggs,Charles W. Archer +16 more
TL;DR: It is proposed that a novel cartilage progenitor population resident in human articular cartilage is identified and characterised which will greatly benefit future cell-based cartilage repair therapies due to its ability to maintain chondrogenicity upon extensive expansion unlike full-depth chondrocytes that lose this ability at only seven population doublings.
Journal ArticleDOI
A new chromosomal instability disorder: the Nijmegen breakage syndrome.
Corry M.R. Weemaes,T.W.J. Hustinx,J. M. J. C. Scheres,P. J. J. Van Munster,J. A. J. M. Bakkeren,R. D. F. M. Taalman +5 more
TL;DR: The similarity of the symptoms in the two sibs, the close consanguinity of their parents and the results of the cytogenetic studies in the family favour the hypothesis that the disorder is an inherited one.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Identification of human chromosomes by DNA-binding fluorescent agents
Torbjörn Caspersson,Torbjörn Caspersson,Lore Zech,Lore Zech,Clas B. Johansson,Clas B. Johansson,E.J. Modest,E.J. Modest +7 more
TL;DR: A convenient measuring technique has been developed for the rapid and accurate recording of fluorescence patterns in human metaphase chromosomes that should be particularly valuable for the identification of chromosomes 4–5 and the individual types in the 6–12 group.
Journal ArticleDOI
The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability.
TL;DR: Apart from certain minor but well defined chromosome regions with especially strong fluorescence, which are subject to certain individual variations, the fluorescence patterns were shown to be quite stable and reproducible.
Journal ArticleDOI
Specific Banding Patterns of Human Chromosomes
Maximo E. Drets,Margery W. Shaw +1 more
TL;DR: This method, which is based on treatment of the chromosomes in situ with NaOH, followed by incubation in sodium chloride-trisodium citrate and Giemsa staining, results in highly specific banding patterns in characteristic regions of the chromosome arms.
Journal ArticleDOI
The Y Chromosome in Human Spermatozoa
Peter Barlow,Canio G. Vosa +1 more
TL;DR: It is found that the distal end of the long arm of the Y chromosome in mitotic and meiotic meta-phase nuclei of human cells displays a fluorescence more brilliant than that shown by the other chromosomes when stained with either quinacrine or quINacrine mustard.