Journal ArticleDOI
Thrombopoiesis and Megakaryocyte Colony Stimulating Factor in the Urine of Patients with Aplastic Anaemia
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The urinary extracts from patients with aplastic anaemia and from healthy donors were investigated in vivo and in vitro for their ability to stimulate megakaryopoiesis and platelet production to suggest that TSF and/or MEG‐CSF seems to be different from EPO.Abstract:
Summary The urinary extracts from patients with aplastic anaemia and from healthy donors were investigated in vivo and in vitro for their ability to stimulate megakaryopoiesis and platelet production. There was a significantly higher concentration of thrombopoiesis stimulating factor (TSF) and megakaryocyte colony stimulating factor (MEG-CSF) in the urine from patients with aplastic anaemia than in that from healthy donors. Neuraminidase treatment did not affect the thrombopoietic activity of TSF, whereas coexisting erythropoictin (EPO) in the extract lost its activity in vivo. These findings suggest that TSF and/or MEG-CSF seems to be different from EPO and that the urine from aplastic anaemia patients would be a good source of TSF and MEG-CSF for purification and characterization.read more
Citations
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Journal ArticleDOI
Annotation: THE ORIGIN, DEVELOPMENT AND REGULATION OF MEGAKARYOCYTES
TL;DR: This Annotation considers the progressive differentiation of marrow stem cells into platelets and the regulatory activities involved therein.
Journal ArticleDOI
Purification and partial characterization of a megakaryocyte colony-stimulating factor from human plasma.
TL;DR: Human plasma obtained from patients with hypomegakaryocytic thrombocytopenia contains a factor that promotes megakaryocyte colony formation by normal human marrow cells that appears to be an important regulator of in vitro human megakARYocytopoiesis at the level of the colony-forming unit megakarianocyte.
Journal ArticleDOI
Regulation of human megakaryocytopoiesis. An in vito analysis.
TL;DR: Initial data suggest that megakaryocyte production is stimulated by a factor detectable in aplastic anemia serum that may be distinct from other known hematopoietic stem cell regulators.
Journal ArticleDOI
Spontaneous cytokine overproduction by peripheral blood mononuclear cells from patients with myelodysplastic syndromes and aplastic anemia.
TL;DR: High cytokine levels were observed in hypocellularity and low blast cell counts in the bone marrow, which may suggest that the increase of cytokines may be a reactive response in Hypocellular bone marrow.
Journal ArticleDOI
Characterization of human megakaryocyte colony-stimulating factor in the urinary extracts from patients with aplastic anemia and idiopathic thrombocytopenic purpura
TL;DR: Initial purification of megakaryocyte colony-stimulating factors (CSF) in urinary extracts from patients with aplastic anemia and idiopathic thrombocytopenic purpura support the notion that MEG-CSF is distinct from Epo.
References
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Journal ArticleDOI
Purification of human erythropoietin.
TL;DR: Human erythropoietin, derived from urine of patients with aplastic anemia, has been purified to apparent homogeneity by seven-step procedure, which yielded a preparation with a potency of 70,400 units/mg of protein in 21% yield.
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Growth of mouse megakaryocyte colonies in vitro
TL;DR: Mouse bone marrow and spleen cells formed pure or mixed colonies of up to 80 megakaryocytes in agar cultures after stimulation by medium conditioned by activated mouse lymphoid cells, and peak activity in conditioning medium was associated with the small lymphocyte fractions in mouse spleen.
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On the Mechanism of Erythropoietin-induced Differentiation XIII. THE ROLE OF SIALIC ACID IN ERYTHROPOIETIN ACTION
TL;DR: The data indicate that terminal sialic acid is required for chemical and biological stability but not for action on target cells of the bone marrow, and the asialohormone has enhanced activity towards the target cells.
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Induction of megakaryocyte colonies with platelet formation in vitro.
TL;DR: The development of megakaryocyte colonies with extensive platelet production in plasma cultures seeded with mouse bone marrow cells and containing either sheep or human erythropoietin in the medium is reported.
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Regulation of Megakaryopoiesis in Long-Term Murine Bone Marrow Cultures
Neil Williams,Heather Jackson,A. P. C. Sheridan,Martin J. Murphy,A. Elste,Malcolm A.S. Moore +5 more
TL;DR: It is shown that no inductive regulator of in vitro clones of megakaryocytes was present in the supernatants from the long-term marrow cultures and that at least two factors were necessary for the induction ofmegakaryocyte progenitors to proliferate and differentiate in semisolid cultures in vitro.