Book ChapterDOI
Transcription and translation.
TLDR
A cDNA encoding the TATA box binding protein, or TATA binding protein (TBP) subunit of transcription factor II D (TFIID), has been identified in the nematode and can substitute for vertebrate TFIID basal activity in in vitro extracts, illustrating a high degree of evolutionary conservation in the basic transcription machinery.Abstract:
Publisher Summary This chapter reviews the current state of the knowledge of transcription and translation in Caenorhabditis elegans. Transcription in eukaryotes is accomplished by three RNA polymerases, each transcribing a particular type of RNA; RNA polymerase I transcribes ribosomal RNA, RNA polymerase II transcribes mRNAs and snRNAs, and RNA polymerase III transcribes tRNAs and other low-molecular-weight RNAs. The chapter focuses on RNA polymerase II transcription. The C. elegans gene for the large subunit of RNA polymerase II has been cloned, and has shown to encode a protein that resembles its vertebrate counterparts. For most polymerase II transcribed genes in C. elegans, a sequence fitting the consensus TATA element about 30 bp upstream of the transcriptional start site is found. Recently, a cDNA encoding the TATA box binding protein, or TATA binding protein (TBP) subunit of transcription factor II D (TFIID), has been identified in the nematode. This protein shows extended regions of sequence similarity to vertebrate TBP, binds to a TATA box sequence and can substitute for vertebrate TFIID basal activity in in vitro extracts. These results illustrate a high degree of evolutionary conservation in the basic transcription machinery.read more
Citations
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References
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The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
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Journal ArticleDOI
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer
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Journal ArticleDOI
Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans
TL;DR: It is demonstrated that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4.
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The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.
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TL;DR: A simple, effective measure of synonymous codon usage bias, the Codon Adaptation Index, is detailed, useful for predicting the level of expression of a gene, for assessing the adaptation of viral genes to their hosts, and for making comparisons ofCodon usage in different organisms.
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A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
Diethard Tautz,Christine Pfeifle +1 more
TL;DR: A non-radioactive in situ hybridization technique for the localization of RNA in whole mount Drosophila embryos and revealed translational control of the maternally derived hb mRNA, which was difficult to detect by conventional techniques.