Translocation of iron citrate and phosphorus in xylem exudate of soybean.
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A modified preculture system, involving 2-day renewals of 0.2 mum FeEDDHA with 3.2, 9.6, or 16 mum P and low levels of other ions, controlled pH depression and produced considerable change in citrate and P levels.Abstract:
Soybean plants, Glycine max (L.) Merrill, in standard solution received 2.5 mum ferric ethylenediamine di(o-hydroxyphenylacetate (FeEDDHA) and 0 to 128 mum phosphorus. Their stem exudates contained: 32 to 52 mum Fe, 120 to 5000 mum P, and 120 to 165 mum citrate. Electrophoresis of exudates with high P caused Fe trailing that precluded identification of any major form of Fe. Exudate with low P gave an anodic band of Fe citrate as the major Fe compound. Phosphate added to exudate in vitro depressed the Fe citrate peak and cause Fe trailing. EDDHA added to exudate in vitro pulled Fe from Fe citrate; citrate then migrated as a slower form and Fe migrated as FeEDDHA. A modified preculture system, involving 2-day renewals of 0.2 mum FeEDDHA with 3.2, 9.6, or 16 mum P and low levels of other ions, controlled pH depression and produced considerable change in citrate and P levels. The exudates contained: 45 to 57 mum Fe, 200 to 925 mum P, and 340 to 1025 mum citrate. The high citrate was from plants grown with low P. The major form of Fe in the exudates was Fe citrate. This is probably the form translocated in the plants.read more
Citations
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Obligatory reduction of ferric chelates in iron uptake by soybeans.
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References
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Journal ArticleDOI
Iron translocation I. Plant culture, exudate sampling, iron-citrate analysis.
TL;DR: Data given in this paper indicate that citrate binds iron in sunflower exudate and suggest that citrates carries iron in intact plants.
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Iron Translocation II. Citrate/Iron Ratios in Plant Stem Exudates
TL;DR: Electrophoretic patterns of iron were obtained despite changes in citrate and iron with time, as iron was raised in the nutrient, the increases of iron in the exudate were proportionately greater than those of citrate.
Journal ArticleDOI
Factors affecting the activity of aconitase.
Sherman R. Dickman,A.A. Cloutier +1 more
Journal ArticleDOI
Translocation of Manganese, Iron, Cobalt, and Zinc in Tomato
TL;DR: Estimates based on metal-citrate equilibrium constants and constants of metal displacement caused by Ca and Mg confirmed that Mn, Co, and Zn were transported predominantly as inorganic cations in the stem exudates.
Journal ArticleDOI
Factors affecting the distribution of iron in plants.
C. H. Rogers,J. W. Shive +1 more