Journal ArticleDOI
A ferric-chelate reductase for iron uptake from soils
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TLDR
The isolation of FRO2 has implications for the generation of crops with improved nutritional quality and increased growth in iron-deficient soils and for the treatment of iron deficiency in plants.Abstract:
Iron deficiency afflicts more than three billion people worldwide, and plants are the principal source of iron in most diets. Low availability of iron often limits plant growth because iron forms insoluble ferric oxides, leaving only a small, organically complexed fraction in soil solutions. The enzyme ferric-chelate reductase is required for most plants to acquire soluble iron. Here we report the isolation of the FRO2 gene, which is expressed in iron-deficient roots of Arabidopsis. FRO2 belongs to a superfamily of flavocytochromes that transport electrons across membranes. It possesses intramembranous binding sites for haem and cytoplasmic binding sites for nucleotide cofactors that donate and transfer electrons. We show that FRO2 is allelic to the frd1 mutations that impair the activity of ferric-chelate reductase. There is a nonsense mutation within the first exon of FRO2 in frd1-1 and a missense mutation within FRO2 in frd1-3. Introduction of functional FRO2 complements the frd1-1 phenotype in transgenic plants. The isolation of FRO2 has implications for the generation of crops with improved nutritional quality and increased growth in iron-deficient soils.read more
Citations
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Journal ArticleDOI
Bacterial iron homeostasis
TL;DR: The expression of the iron homeostatic machinery is subject to iron-dependent global control ensuring that iron acquisition, storage and consumption are geared to iron availability and that intracellular levels of free iron do not reach toxic levels.
Journal ArticleDOI
The Genome of the Diatom Thalassiosira Pseudonana: Ecology, Evolution, and Metabolism
E. Virginia Armbrust,John A. Berges,Chris Bowler,Beverley R. Green,Diego Martinez,Nicholas H. Putnam,Shiguo Zhou,Andrew E. Allen,Andrew E. Allen,Kirk E. Apt,Michael Bechner,Mark A. Brzezinski,Balbir K. Chaal,Anthony Chiovitti,Aubrey K. Davis,Mark S. Demarest,J. Chris Detter,Tijana Glavina,David Goodstein,Masood Z. Hadi,Uffe Hellsten,Mark Hildebrand,Bethany D. Jenkins,Jerzy Jurka,Vladimir V. Kapitonov,Nils Kröger,Winnie W. Y. Lau,Todd W. Lane,Frank W. Larimer,J. Casey Lippmeier,J. Casey Lippmeier,Susan Lucas,Mónica Medina,Anton Montsant,Miroslav Oborník,Miroslav Oborník,Micaela S. Parker,Brian Palenik,Gregory J. Pazour,Paul G. Richardson,Tatiana A. Rynearson,Mak A. Saito,David C. Schwartz,Kimberlee Thamatrakoln,Klaus Valentin,Assaf Vardi,Frances P. Wilkerson,Daniel S. Rokhsar +47 more
TL;DR: The 34 million-base-pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand base-pair mitochondrial genomes were reported in this article.
Book
Heavy metals in soils : trace metals and metalloids in soils and their bioavailability
TL;DR: In this article, the authors defined the sources of heavy metals and metalloids in Soils and derived methods for the determination of Heavy Metals and Metalloids in soil.
Journal ArticleDOI
Biofortification of crops with seven mineral elements often lacking in human diets--iron, zinc, copper, calcium, magnesium, selenium and iodine.
TL;DR: In this paper, the authors review aspects of soil science, plant physiology and genetics underpinning crop bio-fortification strategies, as well as agronomic and genetic approaches currently taken to biofortify food crops with the mineral elements most commonly lacking in human diets: iron (Fe), zinc (Zn), copper (Cu), calcium (Ca), magnesium (Mg), iodine (I) and selenium (Se).
Journal ArticleDOI
IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth
Grégory Vert,Natasha Grotz,Fabienne Dédaldéchamp,Frédéric Gaymard,Mary Lou Guerinot,Jean-François Briat,Catherine Curie +6 more
TL;DR: Genetic evidence is presented that IRT1 is essential for the uptake of iron from the soil, and it is shown that I RT1 is expressed in the external cell layers of the root, specifically in response to iron starvation.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Journal ArticleDOI
RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes
Andrew F. Bent,Barbara N. Kunkel,Douglas Dahlbeck,Kit L. Brown,Renate Schmidt,Jérôme Giraudat,Jeffrey Leung,Brian J. Staskawicz +7 more
TL;DR: The function of the RPS2 gene product in defense signal transduction is postulated to involve nucleotide triphosphate binding and protein-protein interactions and may also involve the reception of an elicitor produced by the avirulent pathogen.
Journal ArticleDOI
Iron: Nutritious, Noxious, and Not Readily Available.
Mary Lou Guerinot,Y. Yi +1 more
TL;DR: The problem that soil-based organisms have with iron is not one of abundance, since iron ranks fourth among a11 elements on the surface of the earth, but rather one of availability in aerobic environments at biological pH, which is far below that required for the optimal growth of plants or microbes.