Transmembrane Transport of Bicarbonate Unravelled
Luis Martínez-Crespo,Sarah H. Hewitt,Nicola Alessandro De Simone,Vladimír Šindelář,Anthony P. Davis,Stephen J. Butler,Hennie Valkenier +6 more
TLDR
In this article, the authors present an assay that allows the kinetics of bicarbonate transport into liposomes to be monitored directly and with great sensitivity, using an encapsulated europium(III) complex.Abstract:
Anion receptors can be used to transport ions across lipid bilayers, which has potential for therapeutic applications. Synthetic bicarbonate transporters are of particular interest, as defects in transmembrane transport of bicarbonate are associated with various diseases. However, no convenient method exists to directly observe bicarbonate transport and study the mechanisms involved. Here, an assay is presented that allows the kinetics of bicarbonate transport into liposomes to be monitored directly and with great sensitivity. The assay utilises an encapsulated europium(III) complex, which exhibits a large increase in emission intensity upon binding bicarbonate. Mechanisms involving CO2 diffusion and the dissipation of a pH gradient are shown to be able to lead to an increase in bicarbonate concentration within liposomes, without transport of the anion occurring at all. By distinguishing these alternative mechanisms from actual bicarbonate transport, this assay will inform the future development of bicarbonate transporters.read more
Citations
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Journal ArticleDOI
Supramolecular chemistry in lipid bilayer membranes.
TL;DR: The current state of the art of supramolecular systems, including receptors, catalysts, signal transducers and ion transporters, and prospects for the future in both fundamental science and applications in biology and medicine are taken.
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Pnictogen-Bonding Catalysis and Transport Combined: Polyether Transporters Made In Situ
Heorhii Humeniuk,Andrea Gini,Xiaoyu Hao,Filipe Diogo De Sousa Coelho,Naomi Sakai,Stefan Matile +5 more
TL;DR: In this paper, anion transport and catalysis with pnictogen and tetrel bonds are combined with polyether cascade cyclizations into bioinspired cation transporters.
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Ion Channels and Transporters as Therapeutic Agents: From Biomolecules to Supramolecular Medicinal Chemistry
TL;DR: Progress that has been made over the last five years on ion channels and transporters is discussed, touching upon biomolecules and synthetic supramolecules that are relevant to biological use, and the identification of therapeutic opportunities for future exploration is concluded.
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Perchlorate Solid-Contact Ion-Selective Electrode Based on Dodecabenzylbambus[6]uril
TL;DR: In this article , Dodecabenzylbambusuril (Bn12BU) was used as an ionophore in the ion-selective membrane (ISM) to develop ion selective electrodes (ISEs) for determination of perchlorate concentration utilizing the poly(3,4-ethylenedioxythiophene) (PEDOT) polymer film as a solid-contact material.
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Transmembrane Transport of Bicarbonate by Anion Receptors
TL;DR: An overview of the synthetic receptors that have been studied as bicarbonate transporters, together with the different assays used to perform transport studies in large unilamellar vesicles, are presented in this paper .
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