Trichoderma lixii (IIIM-B4), an endophyte of Bacopa monnieri L. producing peptaibols.
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TLDR
Optimization studies were carried out on T. lixii (IIIM-B4) using different growth media through Intact Cell Mass Spectrometry (ICMS) and a multifold increase was obtained in production of 11 residue peptaibols using rose bengal medium, which was found to have antifungal activity against Candida albicans.Abstract:
Exploration of microbes isolated from north western Himalayas for bioactive natural products. A strain of Trichoderma lixii (IIIM-B4) was isolated from Bacopa monnieri L. The ITS based rDNA gene sequence of strain IIIM-B4 displayed 99% sequence similarity with different Trichoderma harzianum species complex. The highest score was displayed for Hypocrea lixii strain FJ462763 followed by H. nigricans strain NBRC31285, Trichoderma lixii strain CBS 110080, T. afroharzianum strain CBS124620 and Trichoderma guizhouense BPI:GJS 08135 respectively. Position of T. lixii (IIIM-B4) in phylogenetic tree suggested separate identity of the strain. Microbial dynamics of T. lixii (IIIM-B4) was investigated for small peptides. Medium to long chain length peptaibols of 11 residue (Group A), 14 residue (Group B) and 17 residue (Group C) were identified using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometer. Optimization is undeniably a desideratum for maximized production of desirable metabolites from microbial strain. Here optimization studies were carried out on T. lixii (IIIM-B4) using different growth media through Intact Cell Mass Spectrometry (ICMS). A multifold increase was obtained in production of 11 residue peptaibols using rose bengal medium. Out of these, one of them named as Tribacopin AV was isolated and sequenced through mass studied. It was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH. It was found to have antifungal activity against Candida albicans (25 μg/mL MIC). In this study, we isolated a strain of T. lixii (IIIM-B4) producing medium and long chain peptaibols. One of them named as Tribacopin AV was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH, which had antifungal properties.read more
Citations
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The 18mer peptaibols from Trichoderma virens elicit plant defence responses. Appl Soil Ecol 8:737-746
TL;DR: In this paper, a reverse transcriptase PCR analysis was performed on the trichoderma virens strain Gv29-8, a well-known biocontrol agent and inducer of plant defence responses, produces three lengths of peptaibols, 11, 14 and 18 residues long, with several isoforms of each.
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Bioremediation mechanism and potential of copper by actively growing fungus Trichoderma lixii CR700 isolated from electroplating wastewater.
Vinay Kumar,Sanjay K. Dwivedi +1 more
TL;DR: T. lixii CR700 uses simultaneous surface sorption and accumulation mechanism in Cu2+ removal and can be potentially applied for bioremediation of Cu2- contaminated wastewater in ecofriendly, safe and sustainable way.
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Therapeutic agents from endophytes harbored in Asian medicinal plants
TL;DR: The primary objective of this review is to provide an insight of symbiotic relationship between the endophytes and medicinal plants, specifically belonging to Asia, and also to give detailed information about the novel bioactive secondary metabolites produced from endophyte, that serve as potential therapeutic agents.
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The Emericellipsins A-E from an Alkalophilic Fungus Emericellopsis alkalina Show Potent Activity against Multidrug-Resistant Pathogenic Fungi.
A. E. Kuvarina,Irina A. Gavryushina,Alexander B. Kulko,Igor Ivanov,Eugene A. Rogozhin,M. L. Georgieva,V. S. Sadykova +6 more
TL;DR: In this article, the authors reported the complete primary structure and detailed biological activity for the newly synthesized nonribosomal antimicrobial peptides called emericellipsins B-E.
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