Two independent approaches converge to the cloning of a new <i>Leptosphaeria maculans</i> avirulence effector gene, <i>AvrLmS‐Lep2</i>
Andrey Tikhonov,1Ibodullayev Sardor Nasriddin o'g'li , 2Abdullayev Yunus Yusup o'g'li, 3Sodiqjonov Yaxyobek … +1 more
TLDR
In this article , the same authors reported the cloning of AvrLmS and AvRLep2 using two independent methods: a biparental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach.Abstract:
Brassica napus (oilseed rape, canola) seedling resistance to Leptosphaeria maculans, the causal agent of blackleg (stem canker) disease, follows a gene-for-gene relationship. The avirulence genes AvrLmS and AvrLep2 were described to be perceived by the resistance genes RlmS and LepR2, respectively, present in B. napus 'Surpass 400'. Here we report cloning of AvrLmS and AvrLep2 using two independent methods. AvrLmS was cloned using combined in vitro crossing between avirulent and virulent isolates with sequencing of DNA bulks from avirulent or virulent progeny (bulked segregant sequencing). AvrLep2 was cloned using a biparental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach. Taking these two approaches independently, we found that AvrLmS and AvrLep2 are the same gene. Complementation of virulent isolates with this gene confirmed its role in inducing resistance on Surpass 400, Topas-LepR2, and an RlmS-line. The gene, renamed AvrLmS-Lep2, encodes a small cysteine-rich protein of unknown function with an N-terminal secretory signal peptide, which is a common feature of the majority of effectors from extracellular fungal plant pathogens. The AvrLmS-Lep2/LepR2 interaction phenotype was found to vary from a typical hypersensitive response through intermediate resistance sometimes towards susceptibility, depending on the inoculation conditions. AvrLmS-Lep2 was nevertheless sufficient to significantly slow the systemic growth of the pathogen and reduce the stem lesion size on plant genotypes with LepR2, indicating the potential efficiency of this resistance to control the disease in the field. read more
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A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins
TL;DR: In this paper , a 3D model of AvrLm5-9 was obtained based on the crystal structure of Ecp11-1, a homologous AVR effector candidate from Fulvia fulva.
Journal ArticleDOI
Two independent approaches converge to the cloning of a new <i>Leptosphaeria maculans</i> avirulence effector gene, <i>AvrLmS‐Lep2</i>
Andrey Tikhonov,1Ibodullayev Sardor Nasriddin o'g'li , 2Abdullayev Yunus Yusup o'g'li, 3Sodiqjonov Yaxyobek … +1 more
TL;DR: In this article , the same authors reported the cloning of AvrLmS and AvRLep2 using two independent methods: a biparental cross of avirulent and virulent L. maculans isolates and a classical map-based cloning approach.
Journal ArticleDOI
Genomic Variations and Mutational Events Associated with Plant–Pathogen Interactions
TL;DR: This review focuses on genetic variations and mutational events associated with plant–pathogen interactions and discusses how these genome compartments enhance plants’ and pathogens’ evolutionary processes.
Journal ArticleDOI
Molecular Interactions Between Leptosphaeria maculans and Brassica Species.
TL;DR: A review of the current status of the host-pathogen interaction and highlights some of the future gaps that need addressing moving forward is provided in this article , with a focus on the impact of the B. napus-L. maculans interaction.
Journal ArticleDOI
Beyond the genomes of Fulvia fulva (syn. Cladosporium fulvum) and Dothistroma septosporum: New insights into how these fungal pathogens interact with their host plants
Carl H. Mesarich,Irene Barnes,Ellie Bradley,Silvia de la Rosa,Pierre J. G. M. de Wit,Yan-mei Guo,Scott A. Griffiths,Richard C. Hamelin,Matthieu H. A. J. Joosten,Mengmeng Lu,Hannah M McCarthy,Christiaan Schol,Ioannis Stergiopoulos,Mariana Tarallo,Alex Z. Zaccaron,Rosie E. Bradshaw +15 more
TL;DR: In this paper , the first genome sequences of Fulvia fulva and Dothistroma septosporum were published and significant advances have been made in unravelling their genome architectures.
References
More filters
Journal ArticleDOI
The Sequence Alignment/Map format and SAMtools
Heng Li,Bob Handsaker,Alec Wysoker,T. J. Fennell,Jue Ruan,Nils Homer,Gabor T. Marth,Gonçalo R. Abecasis,Richard Durbin +8 more
TL;DR: SAMtools as discussed by the authors implements various utilities for post-processing alignments in the SAM format, such as indexing, variant caller and alignment viewer, and thus provides universal tools for processing read alignments.
Journal ArticleDOI
Trimmomatic: a flexible trimmer for Illumina sequence data
TL;DR: Timmomatic is developed as a more flexible and efficient preprocessing tool, which could correctly handle paired-end data and is shown to produce output that is at least competitive with, and in many cases superior to, that produced by other tools, in all scenarios tested.
Journal ArticleDOI
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
Aaron McKenna,Matthew Hanna,Eric Banks,Andrey Sivachenko,Kristian Cibulskis,Andrew Kernytsky,Kiran V. Garimella,David Altshuler,Stacey Gabriel,Mark J. Daly,Mark A. DePristo +10 more
TL;DR: The GATK programming framework enables developers and analysts to quickly and easily write efficient and robust NGS tools, many of which have already been incorporated into large-scale sequencing projects like the 1000 Genomes Project and The Cancer Genome Atlas.
Journal ArticleDOI
BEDTools: a flexible suite of utilities for comparing genomic features
Aaron R. Quinlan,Ira M. Hall +1 more
TL;DR: A new software suite for the comparison, manipulation and annotation of genomic features in Browser Extensible Data (BED) and General Feature Format (GFF) format, which allows the user to compare large datasets (e.g. next-generation sequencing data) with both public and custom genome annotation tracks.
Journal ArticleDOI
The variant call format and VCFtools
Petr Danecek,Adam Auton,Gonçalo R. Abecasis,Cornelis A. Albers,Eric Banks,Mark A. DePristo,Robert E. Handsaker,Gerton Lunter,Gabor T. Marth,Stephen T. Sherry,Gilean McVean,Richard Durbin +11 more
TL;DR: VCFtools is a software suite that implements various utilities for processing VCF files, including validation, merging, comparing and also provides a general Perl API.