Journal ArticleDOI
Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses
Reads0
Chats0
TLDR
Degenerate PCR primers were designed to anneal to highly conserved nucleotide sequences identified in the genomes of 10 whitefly-transmitted geminiviruses, and tested for their effectiveness in the amplification of viral DNA fragments from the DNA-A and/or DNA-B components of 15 previously uncharacterized geminIViruses.Abstract:
Geminiviruses are widely recognised as a serious threat to vegetable production in many tropical and subtropical regions. This has increased the need for accurate identification of these viruses. Geminiviruses are well suited to polymerase chain reaction (PCR) methods because they replicate via a double-stranded, circular DNA form. Degenerate PCR primers were designed to anneal to highly conserved nucleotide sequences identified in the genomes of 10 whitefly-transmitted geminiviruses. The PCR primers were tested for their effectiveness in the amplification of viral DNA fragments from the DNA-A and/or DNA-B components of 15 previously uncharacterized geminiviruses from the Americas, the Caribbean Basin, and Africa (.)read more
Citations
More filters
Journal ArticleDOI
Exploiting chinks in the plant's armor: evolution and emergence of geminiviruses
TL;DR: The geminiviruses represent a family of DNA viruses that has circumvented these impediments to emerge as one of the most successful viral pathogens, causing severe economic losses to agricultural production worldwide.
Journal ArticleDOI
The Emergence Of Whitefly-Transmitted Geminiviruses in Tomato in the Western Hemisphere
Journal ArticleDOI
Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination.
TL;DR: UgV isolates were detected in severely mosaic-affected plants from all 11 widely separated locations sampled, and the probable role of recombination in geminivirus evolution in the short to medium term is discussed.
Journal ArticleDOI
A simple method for cloning the complete begomovirus genome using the bacteriophage φ29 DNA polymerase
Alice K. Inoue-Nagata,Leonardo C. Albuquerque,Leonardo C. Albuquerque,Wesley B. da Rocha,Wesley B. da Rocha,Tatsuya Nagata +5 more
TL;DR: The use of this enzyme simplified the cloning steps and increased the cloning efficiency of the complete genome of a circular plant DNA virus.
Journal ArticleDOI
Functional analysis of proteins involved in movement of the monopartite begomovirus, Tomato yellow leaf curl virus.
Maria R. Rojas,Hao Jiang,Raquel Salati,Beatriz Xoconostle-Cázares,Mysore R. Sudarshana,William J. Lucas,Robert L. Gilbertson +6 more
TL;DR: Investigation of the functional properties of proteins potentially involved with movement of the monopartite begomovirus, Tomato yellow leaf curl virus (TYLCV), established that TYLCV infection was limited to phloem cells of shoot apical, leaf, stem, and floral tissues, and the V1 and/or C4 may be analogs of the bipartite beggingomov virus BC1 that have evolved to mediate TY LCV movement withinPhloem tissue.