Journal ArticleDOI
Visualization of the membrane engineering concept: evidence for the specific orientation of electroinserted antibodies and selective binding of target analytes
Anna Kokla,Petros Blouchos,Evangelia Livaniou,Christos Zikos,Sotiris E. Kakabakos,Panagiota S. Petrou,Spyridon Kintzios +6 more
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TLDR
It was proven, with the aid of fluorescence microscopy, that (i) membrane‐engineered cells incorporated the specific antibodies in the correct orientation and that (ii) the inserted antibodies are selectively interacting with the homologous target molecules.Abstract:
Membrane engineering is a generic methodology for increasing the selectivity of a cell biosensor against a target molecule, by electroinserting target-specific receptor-like molecules on the cell surface Previous studies have elucidated the biochemical aspects of the interaction between various analytes (including viruses) and their homologous membrane-engineered cells In the present study, purified anti-biotin antibodies from a rabbit antiserum along with in-house prepared biotinylated bovine serum albumin (BSA) were used as a model antibody-antigen pair of molecules for facilitating membrane engineering experiments It was proven, with the aid of fluorescence microscopy, that (i) membrane-engineered cells incorporated the specific antibodies in the correct orientation and that (ii) the inserted antibodies are selectively interacting with the homologous target molecules This is the first time the actual working concept of membrane engineering has been visualized, thus providing a final proof of the concept behind this innovative process In addition, the fluorescence microscopy measurements were highly correlated with bioelectric measurements done with the aid of a bioelectric recognition assay Copyright © 2013 John Wiley & Sons, Ltdread more
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Development of a Portable, Ultra-Rapid and Ultra-Sensitive Cell-Based Biosensor for the Direct Detection of the SARS-CoV-2 S1 Spike Protein Antigen.
TL;DR: The proof-of-concept development of a biosensor able to detect the SARS-CoV-2 S1 spike protein expressed on the surface of the virus is reported, offering a possible solution for the timely monitoring and eventual control of the global coronavirus pandemic.
Journal ArticleDOI
Lipid vesicles in pulsed electric fields: Fundamental principles of the membrane response and its biomedical applications.
TL;DR: How research on cell-size GUVs as model cell systems has provided novel insight into the basic mechanisms of cell electroporation and associated phenomena is discussed, along with a thorough overview of how electropation and electrofusion have been used as versatile methods to manipulate vesicles of all sizes in different biomedical applications.
Journal ArticleDOI
Biosensors for the Determination of SARS-CoV-2 Virus and Diagnosis of COVID-19 Infection
M. V. Drobysh,Almira Ramanaviciene,Roman Viter,Chien-Fu Chen,Urte Samukaite-Bubniene,Vilma Ratautaite,Arunas Ramanavicius +6 more
TL;DR: Monitoring and tracking infection is required in order to reduce the spread of the coronav virus disease 2019 (COVID-19), induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), to achieve the development and deployment of quick, accurate, and sensitive diagnostic methods.
Journal ArticleDOI
Nanobiosensors for the Detection of Novel Coronavirus 2019-nCoV and Other Pandemic/Epidemic Respiratory Viruses: A Review.
Badriyah Alhalaili,Ileana Nicoleta Popescu,O. Kamoun,Feras Alzubi,Sami Alawadhia,Ruxandra Vidu +5 more
TL;DR: The newest detection methods of the influenza virus responsible for acute respiratory syndrome were compared with conventional methods, highlighting the newest trends in diagnostics, applications, and challenges of SARS-CoV-2 (COVID-19 causative virus) nanobiosensors.
Journal ArticleDOI
Commercially available chemicals as immunizing haptens for the development of a polyclonal antibody recognizing carbendazim and other benzimidazole-type fungicides
Christos Zikos,Alexandra Evangelou,Chrysoula-Evangelia Karachaliou,Georgia Gourma,Petros Blouchos,Georgia Moschopoulou,Constantinos P Yialouris,John Griffiths,Graham Johnson,Panagiota S. Petrou,Sotirios E. Kakabakos,Spyridon Kintzios,Evangelia Livaniou +12 more
TL;DR: The new antibody may be exploited in immunoanalytical systems for detecting benzimidazole-type pesticides e.g. in samples of environmental interest and is being tested as a biorecognition element in the novel FOODSCAN cell biosensor platform for pesticide residue detection based on the Bioelectric Recognition Assay technology.
References
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Gene transfer into mouse lyoma cells by electroporation in high electric fields.
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