Showing papers in "Biosensors and Bioelectronics in 2001"

TL;DR: In this article, the authors present a set of performance criteria for single-use biosensors, i.e., a device that is both disposable after one measurement, and unable to monitor the analyte concentration continuously or after rapid and reproducible regeneration.

TL;DR: Development, characterization and applications of organophosphorus hydrolase-based potentiometric, amperometric and optical biosensors are reviewed.

TL;DR: Skin irritation, characterized by erythema and edema, was either non-existent or mild in >90% of subjects and resolved in virtually all subjects without treatment in several days.

TL;DR: A new amplification strategy was introduced for improving the sensitivity of impedance measurements using biotin labeled protein-streptavidin network complex based on the construction of a cross-linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex.

TL;DR: A system for amperometric detection of Escherichia coli based on the integration of microelectromechanical systems, self-assembled monolayers (SAMS), DNA hybridization, and enzyme amplification with high specificity for E. coli and high sensitivity for Bordetella bronchiseptica was developed.

TL;DR: A novel glucose biosensor based on capacitive detection has been developed using molecularly imprinted polymers and the stability and reproducibility of the biosensor were investigated.

TL;DR: Various applications relating to enzyme activity measurements, clinical monitoring, process monitoring, multianalyte determination, hybrid sensing, environmental monitoring, non-aqueous measurements and other miscellaneous applications are described.

TL;DR: Using this separator, cells or cell fragments and magnetic beads bonded with protein or enzyme suspended in bio-buffer solutions can be successfully separated from their suspensions, envisaging a filterless bio-separator.

TL;DR: A new approach to the rational design of a molecularly imprinted polymer (MIP) selective for creatinine was developed using computer simulation, which demonstrated superior selectivity in comparison to the polymer prepared using traditional approach.

TL;DR: The use of cell-based biosensors outside of the laboratory has been limited due to many issues including preparation of the sample, maintenance of the biological environment, and integration of the electronics for data collection and analysis with the development of an integrated silicon-polydimethylsiloxane cell-cartridge.

TL;DR: The KinExA 3000 instrument and the Cd(II)-specific antibody were used to construct a prototype assay that could correctly assess the concentration of cadmium spiked into a groundwater sample.

TL;DR: In this article, the authors used lithographic technologies to confine neurons to highly defined patterns (40 μm wide stripes) and found that these patterns gave a measure of control over the local density of neurons (100-500 cells/mm2).

TL;DR: It was shown that the MIP-coated microplates are particularly useful for assay development and make MIP coating an attractive alternative to conventional antibodies or receptors used in enzyme linked immunosorbent assay (ELISA).

TL;DR: It is concluded that the microgravimetric DNA sensor with its direct detection of amplified products provide a rapid, low cost and convenient diagnostic method for genetic disease.

TL;DR: In this paper, the analysis of formation, deposition and characterization of cholesterol oxidase (COX) layer-by-layer films were performed and the electrochemical redox processes of the enzyme-layered films deposited either on platinum or ITO coated glass plate were investigated.

TL;DR: Only gold electrodes with adsorbed recombinant HRP forms exhibited high and stable current response to H( 2)O(2) due to its bioelectrocatalytic reduction based on direct electron transfer between gold and HRP, giving strong evidence that the electrode currents are diffusion limited.

TL;DR: Two different microsystems designed for multisite monitoring of the physiological state of a cell population are presented and one of them has been designed to monitor the electrical activity of neurons.

TL;DR: An ISFET based sensor structure which is able to measure both pH and oxygen partial pressure is developed and tested in vitro for simultaneous records of cellular acidification and respiration rates at the same site within the cell culture.

TL;DR: Ultraensitive QCM sensors with resonant frequencies from 39 to 110 MHz for measurements in the liquid phase are presented, based on chemically milled shear mode quartz crystals which are etched only in the center of the blank, forming a thin quartz membrane with a thick, mechanically stable outer ring.

TL;DR: A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid showed a selective response to ganglioside of which sIALic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of gangliosiside.

TL;DR: Future development of an 'electronic canary', analogous to the original canary in the coal mine, could draw upon advances in signal processing and communication to establish a network of sensors in a watershed and to provide useful real-time information on water quality.

TL;DR: The results indicate that the EC QCM biosensor can be used for the study of EC attachment and to detect EC cytoskeletal alterations, and suggest the potential of this cellular biosensor for the real time identification or screening of all classes of biologically active drugs or biological macromolecules that affect cellular attachment, regardless of their molecular mechanism of action.

TL;DR: A microbial biosensor based on a carbon paste electrode containing genetically engineered cells expressing organophosphorus hydrolase (OPH) had excellent storage stability, retaining 100% of its original activity when stored at 4 degrees C for up to 45 days.

TL;DR: A good correlation between the total electrode resistances and the electron-transfer resistances at the conducting supports are found and chronopotentiometry is suggested as a rapid transduction means.

TL;DR: In this research, high speed PCR was performed using a silicon-based microchamber array and three heat blocks, fabricated by semiconductor microfabrication techniques to reduce the thermal cycle time to 1/10 of a commercial PCR instrument.

TL;DR: Results demonstrate a need for multivariate analysis of network activity changes to yield accurate predictions of compound effects and suggest that TMPP acts by a competitive antagonism of GABA inhibition, and are consistent with reported patch-clamp analysis of T MPP-induced reduction of inhibitory postsynaptic current amplitudes.

TL;DR: A new technique is presented for in-vivo remote query measurement of the complex permittivity spectra of a biological culture solution through wireless monitoring of a sensor comprised of a printed inductor-capacitor resonant-circuit within the culture solution of interest.

TL;DR: In this paper, an electrochemical biosensor for cow's milk progesterone was developed and used in a competitive immunoassay under thin-layer, continuous-flow conditions, which was fabricated by depositing anti-progesterone monoclonal antibody (mAb) onto screen-printed carbon electrodes (SPCEs).

TL;DR: Enzyme solution-oxidized porous silicon-crystalline silicon structure was used to detect changes in pH during the hydrolysis of tributyrin as a shift in the capacitance-voltage (C-V) characteristics.

TL;DR: In this study coated paramagnetic particles were used as a physical label in a non-competitive solid phase "sandwich" assay for the detection of human transferrin and Quantitation of the paramagnetic label was achieved using an electronic detection system.