Open AccessJournal Article
Vitamins C, E and lipid peroxidation levels in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men
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TLDR
The level of MDA in seminal plasma and spermatozoa and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men and their relationships with semen parameters may be a diagnostic tool for etiology of infertility in the asthenOTzoosPermic patients.Abstract:
Background: It has been shown that reactive oxygen species (ROS) can lead to deleterious effects on a range of sperm parameters. Vitamins E and C are capable of reducing ROS levels and lipid peroxidation. Objective: The aim of study was to determine the level of lipid peroxidation as indicated by Malondialdehyde (MDA) and vitamins E and C in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men and their relationships with semen parameters. Materials and Methods: Forty men with normozoospermic and 60 infertile men with asthenoteratozoospermic semen profiles were randomly selected. Semen analysis was done according to the WHO standard. Sex hormonal profiles were measured by ELISA methods. The level of vitamins (C and E) and MDA were measured by HPLC and tiobarbiotic acid, respectively. Results: MDA concentration in the spermatozoa (0.1±0.06 nmol/ml) and seminal plasma (1.9±0.35 nmol/ml) of asthenoteratozoospermic were significantly higher than in normozoospermic males (p<0.001).The level of vitamins E and C in seminal plasma of normozoospermic were significantly higher than in asthenoteratozoospermic males (p<0.01). However, the amount of vitamin C in the spermatozoa of normozoospermic was significantly less than in asthenoteratozoospermic males (p<0.01). Sensitivity, specificity, positive and negative prognostic value of MDA of seminal plasma and spermatozoa were more than vitamins C and E. The level of vitamin C of spermatozoa had more diagnostic value when compare to vitamins C and E in seminal plasma. Conclusion: The level of MDA in seminal plasma and spermatozoa and vitamin C in spermatozoa may be a diagnostic tool for etiology of infertility in the asthenoteratozoospermic patients.read more
Citations
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Reactive oxygen species and antioxidant in seminal plasma and their impact on male fertility
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Is male infertility associated with increased oxidative stress in seminal plasma? A-meta analysis.
Chao Huang,Xiyue Cao,Dejiang Pang,Chao Li,Qihui Luo,Yuanfeng Zou,Bin Feng,Lixia Li,Anchun Cheng,Zhengli Chen +9 more
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