Journal ArticleDOI
Withanolide synthesis in cultures of Withania somnifera transformed with Agrobacterium tumefaciens
Swagata Ray,Sumita Jha +1 more
TLDR
Transformed organ cultures of Withania somnifera established following infection with wild type nopaline and octopine strains of Agrobacterium tumefaciens found shooty teratomas of altered phenotype spontaneously developed, finding the main difference was found in the nature of the galls formed and in their subsequent morphological competence.About:
This article is published in Plant Science.The article was published on 1999-08-13. It has received 38 citations till now. The article focuses on the topics: Withania somnifera & Nopaline.read more
Citations
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Journal ArticleDOI
Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A.
TL;DR: The optimized conditions for biomass accumulation and withanolide A production were found to be 10 g L(-1) of the inoculum on fresh weight basis, the full strength MS medium, 3% (w/v) sucrose, four weeks culture period and the initial medium pH of 5.8.
Journal ArticleDOI
Withanolide A biogeneration in in vitro shoot cultures of ashwagandha (Withania somnifera DUNAL), a main medicinal plant in Ayurveda.
Rajender S. Sangwan,Narayan D. Chaurasiya,Payare Lal,Laxminarain Misra,G. C. Uniyal,Rakesh Tuli,Neelam S. Sangwan +6 more
TL;DR: The enhanced de novo biogenesis of withanolide A in shoot cultures was corroborated with radiolabel incorporation studies using [2-(14)C] acetate as a precursor and production of withaferin A was also found in the in vitro shoot cultures.
Journal ArticleDOI
Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.
TL;DR: The first report of the presence of withasteroids in undifferentiated callus cultures of W. somnifera is reported, and this is the first detection of withaferin A in the roots of the plant.
Book
Plant Cell and Tissue Culture - A Tool in Biotechnology: Basics and Application
TL;DR: In this paper, the authors present a survey of the development of cell and tissue cultures and their application in the field of fertility management, including the use of stem cells in fertility management.
Journal ArticleDOI
A Decade of Molecular Understanding of Withanolide Biosynthesis and In vitro Studies in Withania somnifera (L.) Dunal: Prospects and Perspectives for Pathway Engineering.
Niha Dhar,Sumeer Razdan,Satiander Rana,Wajid Waheed Bhat,Ram A. Vishwakarma,Surrinder K. Lattoo +5 more
TL;DR: A detailed overview of various studies undertaken by the authors and collated literature related to molecular and in vitro approaches employed in W. somnifera for understanding various molecular network interactions in entirety is provided.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid
TL;DR: The interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon are reported, which allows introduction of the manipulated T- DNA into plant cells.
Book
Indigenous Drugs Of India
TL;DR: The Textbook of Dietetics by Prof. Stanley Davidson and Dr. I. Anderson provides an admirable guide to the science and practice of the subject and is a reminder of how fundamentally the authors' ideas on diet in health and disease have been modified in the last twenty years.
Journal ArticleDOI
Fingerprints of Agrobacterium Ti plasmids.
TL;DR: Three groups of Ti plasmids are discernible based upon both their genetic characteristics and their Sma I fingerprints, which confer oncogenicity and octopine utilization on the bacterium.
Journal ArticleDOI
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
TL;DR: In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected and enzyme activities as low as 0.2 micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected.
Related Papers (5)
Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more